Round filter chromatogram

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The round filter chromatogram method (also the chroma method , chroma test , round filter chromatography , English round filter chromatography , circular chromatography or chroma test ) according to Ehrenfried Pfeiffer is an anthroposophical method for the qualitative assessment of plants, food, soil and compost, with the results of which the chromatograms, differences between food, compost or soil samples can be made visible. Together with the rising image method , copper chloride crystallization (biocrystallization) and the drip image method, it is counted among the image-creating methods .

Emergence

The first round filter chromatogram tests were invented by the chemist Friedlieb Ferdinand Runge , who published the collections "Musterbilder" and "Bildungsstrieb derstoffe" as early as 1850 and 1855. These pattern images, also known as Runge images, are regarded as the forerunners of paper chromatography . In 1953, Ehrenfried Pfeiffer developed the round filter chromatogram method from this in order to enable quick orientation about the qualitative condition of soil and compost.

Procedure

A round chromatography paper is impregnated with a silver nitrate solution up to a radius of 4 cm via a central wick . After a subsequent drying time of 2 to 3 hours, the extract of the test material rises in a new wick. The process is stopped as soon as the solution in the paper has spread to a radius of 6 cm. After the pictures have dried, the effect of diffuse light is necessary for the development of the picture. The resulting round filter chromatogram is evaluated using morphological criteria and interpreted with the help of standardized comparison images.

swell

  1. ↑ Uwe Geier, Lothar Seitz: Image optimum and image types in the round filter chromatogram (chroma) when examining plants , Elements of Natural Science 85, 2006, 57-80
  2. ↑ For example in grain breeding research
  3. ↑ Helmut Voitl, Elisabeth Guggenberger: The chroma soil test . ISBN 3-7015-0036-3
  4. ↑ Nicola Haßold-Piezunka: Suitability of the chroma soil test for determining compost quality and degree of rotting . Dissertation University of Oldenburg 2003
  5. ↑ Günther Harsch, Heinz H. Bussmas: Pictures that paint themselves. The chemist Runge and his “sample pictures for friends of the beautiful” . DuMont Buchverlag, Cologne 1985, ISBN 3-7701-1639-9 .
  6. ↑ Ehrenfried Pfeiffer 1959, s. literature
  7. ↑ According to Ehrenfried Pfeiffer, "in order to learn how to interpret the chromatograms, one must first produce comparison images of known, precisely definable substances or preparations, so-called standards, in order to compare the unknown images found". Ehrenfried Pfeiffer 1959, s. literature

literature

  • Ehrenfried Pfeiffer: A qualitative chromatographic method for determining biological values. I. Differences between humus and compost quality . In: Living Earth . No. 5, 1959, ISSN  0023-9917 , pp. 205-215.
  • Ehrenfried Pfeiffer: A qualitative chromatographic method for determining biological values. II. Studies on vitamin preparations . In: Living Earth . No. 6, 1959, ISSN  0023-9917 , pp. 241-249.
  • Ehrenfried Pfeiffer: Chromatographic investigations on seeds, flours, yeasts and their products . In: Living Earth . No. 5, 1960, ISSN  0023-9917 , pp. 204-216.
  • Ehrenfried Pfeiffer: Chromatography applied to Quality Testing . Bio-Dynamic Literature, Wyoming, Rhode Island 02898, 1984.
  • Helmut Voitl, Elisabeth Guggenberger: The chroma soil test . Verlag Orac, Vienna 1986, ISBN 3-7015-0036-3 .
  • Jairo Restrepo Rivera, Sebastião Pinheiro: Cromatografía . coas ediciones, Cali 2011, ISBN 978-958-44-8582-3 .

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