STR analysis

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The STR analysis is a method of DNA analysis to determine short tandem repeats . The STR method is used as a STR profile analysis in criminology, forensic medicine and for paternity proofs.

properties

In the STR analysis, the number of short tandem repeats on several chromosomes is determined by DNA extraction with a subsequent polymerase chain reaction (PCR) . STR are microsatellites between two and thirteen nucleotides in length that occur in the genome hundreds of times in series on each chromosome. A parentage assessment using STR analysis compares two different samples. After the PCR, the amplified products are usually subjected to agarose gel electrophoresis or capillary electrophoresisseparated and proven. STR analysis can also be performed without DNA extraction.

Individual evidence

  1. ↑ A. Ambers, H. Gill-King, D. Dirkmaat, R. Benjamin, J. King, B. Budowle: Autosomal and Y-STR analysis of degraded DNA from the 120-year-old skeletal remains of Ezekiel Harper. In: Forensic science international. Genetics. Volume 9, March 2014, pp. 33-41, ISSN  1878-0326 . doi : 10.1016 / j.fsigen.2013.10.014 . PMID 24528577 .
  2. ^ C. Hohoff, B. Brinkmann: Human identity testing with PCR-based systems. In: Molecular biotechnology. Volume 13, Number 2, December 1999, pp. 123-136, ISSN  1073-6085 . doi : 10.1385 / MB: 13: 2: 123 . PMID 10934527 .
  3. ↑ JM Butler, E. Buel, F. Crivellente, BR McCord: Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysis. In: Electrophoresis. Volume 25, Numbers 10-11, June 2004, pp. 1397-1412, ISSN  0173-0835 . doi : 10.1002 / elps.200305822 . PMID 15188225 .
  4. ^ JE Sim, SJ Park, HC Lee, SY Kim, JY Kim, SH Lee: High-throughput STR analysis for DNA database using direct PCR. In: Journal of forensic sciences. Volume 58, Number 4, July 2013, pp. 989-992, ISSN  1556-4029 . doi : 10.1111 / 1556-4029.12166 . PMID 23683293 .