The Sangurtest is a biochemical procedure for the detection of hemoglobin and myoglobin . The peroxidative effect of hemoglobin and myoglobin is used, under the influence of which the oxidation of a chromogenic substance is catalyzed to a green-blue dye. The Sangur test is a variant of the detection with O-tolidine .
In nephrology , the Sangurtest is used as a rapid test to differentiate between intermittent and constant microhematuria . It is also suitable as part of a family examination in the case of benign family hematuria and Alport's syndrome .
The urine to be examined should be examined as soon as possible after collection, as long periods of standing can lead to haemolysis, so that erythrocyturia would no longer be visible under the microscope. Both free hemoglobin and myoglobin indicate a positive reaction, so the detection of erythrocytes in the urine (> 5 erythrocytes per microliter of urine) must be ensured microscopically .
Residues of strong oxidants (such as cleaning agents) in the urine container can lead to false positive results.
In forensics , the Sangur test is used as a pre-test for detecting traces of blood.
- RV Winchester, Heather Wansbrough: Blood Detection by chemical methods . New Zealand Institute of Chemistry. PDF ( Memento of the original from October 29, 2013 in the Internet Archive ) Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice. .