Split TEV

The Split TEV (, TEV-cleaved ') is a biochemical method of detecting protein-protein interactions in living cells.

principle

The Split TEV is provided as a protein-fragment complementation assay is a method for the detection of binding by complementation of fragments of a reporter protein ( English protein complementation assay , PCA), as well as the bimolecular fluorescence complementation . The split TEV consists of a Nia protease from Tobacco Etch Virus (TEV protease), which has been broken down into two enzymatically inactive parts. One part each (the N -terminal NTEV or the C -terminal CTEV) is attached to the two proteins to be examined as a fusion protein , so that when the two proteins bind, the attached protease parts are reconstituted and the protease becomes enzymatically active.

literature

Michael C Wehr, Lisa Reinecke, Anna Botvinnik, Moritz J Rossner: Analysis of transient phosphorylation-dependent protein-protein interactions in living mammalian cells using split-TEV . In: BMC Biotechnology . 8, 2008, p. 55. doi : 10.1186 / 1472-6750-8-55 . PMID 18620601 . PMC 2483975 (free full text).
Minou S. Djannatian, Sabrina Galinski, Tobias M. Fischer, Moritz J. Rossner: Studying G protein-coupled receptor activation using split-tobacco etch virus assays . In: Analytical Biochemistry . 412, No. 2, 2011, pp. 141-52. doi : 10.1016 / year from 2011.01.042 . PMID 21295005 .

Individual evidence

↑ MC Wehr, R. Laage, U. Bolz, TM Fischer, S. Grünewald, S. Scheek, A. Bach, KA Nave, MJ Rossner: Monitoring regulated protein-protein interactions using split TEV. In: Nature methods. Volume 3, Number 12, December 2006, pp. 985-993, ISSN 1548-7091 . doi : 10.1038 / nmeth967 . PMID 17072307 .

[1] MC Wehr, R. Laage, U. Bolz, TM Fischer, S. Grünewald, S. Scheek, A. Bach, KA Nave, MJ Rossner: Monitoring regulated protein-protein interactions using split TEV. In: Nature methods. Volume 3, Number 12, December 2006, pp. 985-993, ISSN 1548-7091 . doi : 10.1038 / nmeth967 . PMID 17072307 .