Sulfovanillin

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Sulfovanillin and the chemically closely related sulfobenzaldehyde are reagents that can be used to selectively detect different cell structures. The reagents for the determination of fungi from the order of those most frequently Täublingsartigen used (Russulales). They can be used in certain cases Laticiferen , pseudo, macro, and Gloeo Zystiden and stain gloeoplere hyphae. It is one of the most important chemical detection reactions, especially when determining deaflings and milklings . The cells turn more or less blue with a positive reaction with sulfovanillin and gray to blackish with sulfobenzaldehyde. From a chemical point of view, sulfovanillin or sulfobenzaldehyde is a solution of vanillin / benzaldehyde in more or less concentrated sulfuric acid . Usually the finished reagent contains about 70% sulfuric acid. It is believed that the reagent reacts with the sesquiterpenes present in the cells , especially if they have aldehyde functions .

swell

  • Michael J. Larsen, HH Burdsall: A consideration of the term gloeocystidium . In: Memoirs Of The New York Botanical Garden . tape 28 , no. 1 , 1976, p. 123-130 ( PDF ).

Individual evidence

  1. reagents . Association for Mushroom Science Biberist.
  2. The cross with the deafblings - first part. Swiss Association of Official Mushroom Control Bodies.
  3. Lexicon with terms from mycology and microscopy technology .
  4. S. Camazine, AT Lupo Jr .: Labile Toxic Compounds of the Lactarii: The Role of the Laticiferous Hyphae as a Storage Depot for Precursors of Pungent Dialdehydes . In: Mycologia . tape 76 , 1984, pp. 355-358 , doi : 10.2307 / 3793113 , JSTOR : 3793113 .