TBS-T buffer
TBST buffer ( English Tris-buffered saline with Tween 20 , TBST) is an in biochemistry for solution of antibodies and immunoconjugates used buffer .
properties
TBS-T buffer is based on the TBS buffer and is used to examine the binding and storage of antibodies as well as for immunodetection , including in Western Blot , in ELISA , in ELISPOT , in immunofluorescence and in immunohistochemistry . TBS-T buffer mostly consists of 10 - 50 mM TRIS as a buffer substance, 150 mM sodium chloride for a physiological ionic strength and as a surfactant 0.05% (m / V) Tween 20 , occasionally 0.1% (m / V) instead Triton X-100 . The pH is, depending on the protocol with dilute hydrochloric acid adjusted to 7.2 to 7.6. The surfactant contained reduces non-specific interactions when the antibodies or immunoconjugates are used, which results in less coloration of the background. Triton X-100 can interfere with subsequent UV- based detections.
Individual evidence
- ^ A b Academic Press: Antibodies in Cell Biology. Academic Press, 1993, ISBN 978-0-080-85935-4 , p. 99.
- ↑ MA Hayat: Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas. Academic Press, 2005, ISBN 978-0-080-49520-0 , p. 473.
- ↑ John Pound: Immunochemical Protocols. Springer Science & Business Media, 1998, ISBN 978-1-592-59257-9 , p. 411.