Tandem mass day

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Tandem Mass Tag ('tandem atomic mass marking', TMT ) is a biochemical method for the mass spectrometric determination of biomolecules such as proteins , DNA or RNA . The TMT uses, among other things, isobar labeling and tandem mass spectrometry . The molecules to be examined are provided with different markings , which are isobaric (have the same initial mass), but result in fragments of different weights and thus distinguishable (more precisely reporter ions ) in the tandem mass spectrometer .

principle

TMT Zero Day.

There are three variants of the TMT:

  • TMTzero - without an isotope mark on the tag
  • TMTduplex - isobaric pairs after marking with an isotope
  • TMTsixplex - an isobaric set of six mass labels with five different isotopes

The tags used contain four areas, a mass reporter area, a cleavable linker area, a mass normalization area and a reactive group that binds to the biomolecule. The different markings are chemically identical, but have different isotopes in the mass reporter and mass normalization range. When determining the molecular weight by gel electrophoresis , chromatography or simple mass spectrometry, the various markings cannot be distinguished. Only through further fragmentation in tandem mass spectrometry do the individual markings become apparent after the peptide bonds have been broken . The patents for TMT are held by Proteome Sciences Plc . The chemical structure of the various markings is recorded in the Unimod database and can be edited with Mascot .

Individual evidence

  1. a b Thompson A, Schäfer J, Kuhn K et al. : Tandem mass tags: a novel quantification strategy for comparative analysis of complex protein mixtures by MS / MS . In: Anal. Chem. . 75, No. 8, 2003, pp. 1895-904. doi : 10.1021 / ac0262560 . PMID 12713048 .
  2. Dayon L, Hainard A, Licker V, Turck N, Kuhn K, Hochstrasser DF, Burkhard PR, Sanchez JC: Relative quantification of proteins in human cerebrospinal fluids by MS / MS using 6-plex isobaric tags . In: Anal. Chem. . 80, No. 8, 2008, pp. 2921-31. doi : 10.1021 / ac702422x . PMID 18312001 .