Transcription factor IIF
Transcription factor IIF (TFIIF) is a protein complex in the cell nucleus of vertebrate animals that is essential for the initiation of the transcription of all genes . In particular, TFIIF binds to RNA polymerase II and stabilizes the TFIID - TFIIA - TFIIB complex, and thus contributes to the precise positioning of the pre-initiation complex at the beginning of the gene. Further functions of TFIIF during the elongation phase are now known.
TFIIF consists of two subunits (RAP74, RAP30) with 517 and 249 amino acids. The second subunit has helicase activity ( EC 3.6.1.- ). RAP74 binds to FCP1 , which is necessary for the recycling of the polymerase. RAP74 also interacts with TAF6 and RAP30 with HTATSF1 as well as with GPBP1 .
Individual evidence
- ↑ Reinberg / reactome: Recruitment of RNA Polymerase II Holoenzyme by TFIIF to the pol II promoter: TFIID: TFIIA: TFIIB complex ( page no longer available , search in web archives ) Info: The link was automatically marked as defective. Please check the link according to the instructions and then remove this notice.
- ^ Cojocaru M, Jeronimo C, Forget D, et al. : Genomic location of the human RNA polymerase II general machinery: evidence for a role of TFIIF and Rpb7 at both early and late stages of transcription . In: Biochem. J. . 409, No. 1, January 2008, pp. 139-47. doi : 10.1042 / BJ20070751 . PMID 17848138 .
- ↑ Cheng B, Price DH: Properties of RNA polymerase II elongation complexes before and after the P-TEFb-mediated transition into productive elongation . In: J. Biol. Chem. . 282, No. 30, July 2007, pp. 21901-12. doi : 10.1074 / jbc.M702936200 . PMID 17548348 .
- ↑ UniProt P35269 , UniProt P13984
- ↑ Abbott KL, Renfrow MB, Chalmers MJ, et al. : Enhanced binding of RNAP II CTD phosphatase FCP1 to RAP74 following CK2 phosphorylation . In: Biochemistry . 44, No. 8, March 2005, pp. 2732-45. doi : 10.1021 / bi047958h . PMID 15723518 .
