Veronal acetate buffer
The barbital acetate buffer , also veronal acetate buffer or Michaelis buffer , according to Leonor Michaelis is a buffer system that is used in clinical chemistry . It consists of barbital (5,5-diethylbarbituric acid sodium salt ), sodium acetate and hydrochloric acid and buffers in the range from pH 3.0 to pH 7.4.
The veronal buffer for the pH range 6.8 to 9.2 is made from just two stock solutions , a solution of (5,5-diethylbarbituric acid sodium salt ) and a hydrochloric acid solution.
The stock solutions are stable and can be stored for a few months at a temperature of 4 ° C. The finished buffer solution is susceptible to the growth of microorganisms at least at neutral pH values despite being stored at 4 ° C and should be freshly prepared.
The starting substance barbital is subject to the narcotics prescription regulation .
Individual evidence
- ↑ Trade name Veronal
- ↑ Wolf D. Kuhlmann, "Buffer Solutions" , veronal acetate buffer.
- ↑ SE Ruzin: Plant Microtechnology and Microscopy . Oxford University Press, 1999.