Virus isolation

As a virus isolation is defined as the deliberate propagation of viruses from biological samples such. B. infectious material. The viruses can be reproduced in cell cultures , embryonated hen's eggs or test animals and identified and characterized using specific detection methods . Virus isolation is used either as a classic method for discovering new, previously unknown viruses or as a method for detecting a known virus as part of virus diagnostics . Virus isolation contrasts with direct pathogen detection without reproduction.

The process includes the collection of the analysis sample , occasionally also a purification and a determination of the systematics or other properties such as tropism , infectivity and pathogenicity . When growing in animals as the host , the isolated virus can be assigned to a specific clinical picture in accordance with the Henle-Koch postulates .

literature

• Susanne Modrow, Dietrich Falke , Uwe Truyen: Molecular Virology. An introduction for biologists and medical professionals. 2nd Edition. Spectrum textbook, Heidelberg 2002, ISBN 3-8274-1086-X . (with references, English translation 2006) .
• David M. Knipe, Peter M. Howley, et al. (eds.): Fields' Virology . (2 volumes; standard work on virology) 5th edition, Lippincott Williams & Wilkins, Philadelphia 2007, ISBN 978-0-7817-6060-7 .
• HW Doerr, WH Gerlich (ed.): Medical Virology , 2nd edition Stuttgart 2010 pp. 97ff ISBN 978-3-13-113962-7