A431 (cell line)
A431 cells are a model cell line (epidermoid carcinoma ) used in biomedical research and a. is used in cancer research .
In particular, cell cycle and cancer-associated pathways of cellular signal transduction have been investigated with A431 since it has been known that this cell line has abnormally high levels of expression of EGFR-1 (or Her1 / neu); they can therefore be used as a positive control for EGFR expression. A431 do not have a functional p53 protein, a potent tumor suppressor molecule, and are therefore extremely sensitive to mitogenic stimuli. A431 were isolated from the epidermis of an 85 year old female patient.
EGF stimulation of A431 induces rapid tyrosine - phosphorylation of intracellular proteins , cellular processes, such as growth, proliferation and apoptosis regulate.
Treatment of A431 cells with bradykinin reduced basal and EGF-induced EGFR phosphorylation.
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- ^ A431 - Cytokines and Cells Online Pathfinder Encyclopaedia . Archived from the original on February 10, 2013. Retrieved on December 13, 2013.
- ↑ Graness A, Hanke S, Boehmer FD, et al. : Protein-tyrosine-phosphatase-mediated epidermal growth factor (EGF) receptor transinactivation and EGF receptor-independent stimulation of mitogen-activated protein kinase by bradykinin in A431 cells . In: Biochem. J. . 347, No. Pt 2, April 2000, pp. 441-47. doi : 10.1042 / 0264-6021: 3470441 . PMID 10749673 . PMC 1220976 (free full text).