A549 cells
A549 cells (also known as hA549 or A-549 ) are a specified human cell line that is used in molecular biology and virology for cell culture . It comes from an explanted adenocarcinoma of the lungs of a 58-year-old white American. The cells were established in 1972 by Donald J. Giard at MIT . A549 cells are used for pharmacological studies, transfection and virus replication .
A549 cells are hypo triploid with different chromosome occupancy of around 66 and multiple mutations . They synthesize a relatively large amount of lecithin , using the cytidine-diphosphocholine metabolic pathway . The cells grow adherent as a monolayer , RPMI-1640 , Ham's F12K or DMEM is used as the cell culture medium, each with the addition of FCS .
Individual evidence
- ↑ DJ Giard et al .: In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors . J. National Cancer Institute (1973) 51 (5): pp. 1417-1423 PMID 4357758
- ^ KA Foster et al .: Characterization of the A549 cell line as a type II pulmonary epithelial cell model for drug metabolism. Experimental Cell Research (1998) 243 (2): pp. 359-366 PMID 9743595