Andreas Plückthun

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Andreas Plückthun

Andreas Plückthun (born May 7, 1956 ) is a German biochemist whose research focuses on the field of protein engineering . He is a professor at the Biochemical Institute at the University of Zurich . He was elected a member of the European Molecular Biology Organization (EMBO) in 1992 and of the National Academy of Sciences (Leopoldina) in 2003 . recorded. He is also a co-founder of the biotechnology companies MorphoSys AG (Martinsried, Germany), Molecular Partners AG (Zurich-Schlieren, Switzerland) and G7 Therapeutics (Zurich-Schlieren, Switzerland).

Life

Andreas Plückthun was born in Heidelberg (Germany) and studied chemistry at Heidelberg University . He did his doctorate in 1982 at the University of California at San Diego (USA) with Edward Dennis on the subject of "The interfacial catalysis of phospholipase A2". He was then a postdoctoral fellow at Harvard University with Jeremy Knowles , where he worked on the secretion process of beta-lactamase in Escherichia coli . In 1985 he became group leader at the gene center at the Max Planck Institute for Biochemistry in Martinsried (Germany). Since his appointment in 1993 he has been Professor of Biochemistry (Full Professor) at the University of Zurich (Switzerland).

Act

His research laid important foundations for the development of recombinant antibody technology, particularly the use of E. coli to rapidly produce engineered antibodies and studies of synthetic antibodies that led to the first fully synthetic antibody library. His laboratory developed a method for evolution in the test tube , the ribosome display of proteins. The Designed Ankyrin Repeat Proteins (DARPins) were designed by his team as a robust alternative to antibodies ( antibody mimics) . His research on G protein-coupled receptors led to new methods of directed evolution that facilitate structural studies, drug screening and drug design.

Awards

He received the Lecturer Award of the Fonds derchemischen Industrie in 1990. In 2000 he was awarded the Karl-Heinz-Beckurts Preis (Munich). In 2002 he was awarded the JPMorgan Chase Health Award (San Jose, CA, USA), the Wilhelm Exner Medal (Vienna, Austria) and the Grand Prix du Jury (European Grand Prix for Innovation, Monaco). In 2005, together with the other co-founders of Molecular Partners AG , he received the Swiss Technology Award and the WA De Vigier Foundation Prize. In 2011 he was awarded an Advanced Grant from the European Research Council (ERC). In 2016 he was awarded the Christian B. Anfinsen Award from the Protein Society.

Web links

Individual evidence

  1. ↑ Biochemical Institute, University of Zurich
  2. ↑ EMBO membership directory
  3. ↑ Member entry by Prof. Dr. Andreas Plückthun (with picture) at the German Academy of Sciences Leopoldina , accessed on March 15, 2016.
  4. ^ Molecular Partners
  5. ^ G7 Therapeutics
  6. ^ Curriculum vitae, source: Biochemical Institute, University of Zurich
  7. ↑ A. Skerra, A. Plückthun: Assembly of a functional immunoglobulin Fv fragment in Escherichia coli. In: Science. 240 (4855), 1988, pp. 1038-1041. PMID 3285470
  8. ^ R. Glockshuber, M. Malia, I. Pfitzinger, A. Plückthun: A comparison of strategies to stabilize immunoglobulin Fv fragments. In: Biochemistry. 29 (6), 1990, pp. 1362-1367. PMID 2110478
  9. ↑ A. Wörn, A. Plückthun: Stability engineering of antibody single-chain Fv fragments. In: J Mol Biol. 305 (5), 2001, pp. 989-1010. doi: 10.1006 / jmbi.2000.4265 . PMID 11162109
  10. ↑ A. Plückthun, P. Pack: New protein engineering approaches to multivalent and bispecific antibody fragments. In: Immunotechnology. 3 (2), 1997, pp. 83-105. PMID 9237094
  11. ↑ A. Knappik, L. Ge, A. Honegger, P. Pack, M. Fischer, G. Wellnhofer, A. Hoess, J. Wölle, A. Plückthun, B. Virnekäs: Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides. In: J Mol Biol. 296 (1), 2000, pp. 57-86. doi: 10.1006 / jmbi.1999.3444 . PMID 10656818
  12. ↑ J. Hanes, A. Plückthun: In vitro selection and evolution of functional proteins by using ribosome display. In: Proc Natl Acad Sci U.S.A. 94 (10), 1997, pp. 4937-4942. PMID 9144168
  13. ↑ J. Hanes, L. Jermutus, S. Weber-Bornhauser, HR Bosshard, A. Plückthun: Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries. In: Proc Natl Acad Sci U.S.A. 95 (24), 1998, pp. 14130-14135. PMID 9826665
  14. ↑ HK Binz, MT Stumpp, P. Forrer, P. Amstutz, A. Plückthun: Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins. In: J Mol Biol. 332 (2), 2003, pp. 489-503. PMID 12948497
  15. ↑ HK Binz, P. Amstutz, A. Kohl, MT Stumpp, C. Briand, P. Forrer, MG Grütter, A. Plückthun: High-affinity binders selected from designed ankyrin repeat protein libraries. In: Nat Biotechnol. 22 (5), 2004, pp. 575-582. doi: 10.1038 / nbt962 . PMID 15097997
  16. ↑ A. Plückthun: Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy. In: Annu Rev Pharmacol Toxicol. 55 (), 2015, pp. 489-511. doi: 10.1146 / annurev-pharmtox-010611-134654 PMID 25562645
  17. ↑ CA Sarkar, I. Dodevski, M. Kenig, p Dudli, A. Mohr, E. Hermans, A. Pluckthun: Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity. In: Proc Natl Acad Sci US A. 105 (39), 2008, pp. 14808-14813. doi: 10.1073 / pnas.0803103105 . PMID 18812512
  18. ↑ DJ Scott, A. Plückthun: Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells. In: J Mol Biol. 425 (3), 2013, pp. 662-677. doi: 10.1016 / j.jmb.2012.11.015 . PMID 23164568 .
  19. ^ P. Egloff, M. Hillenbrand, C. Klenk, A. Batyuk, P. Heine, S. Balada, KM Schlinkmann, DJ Scott, M. Schütz, A. Plückthun: Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli. In: Proc Natl Acad Sci US A. 111 (6), 2014, pp. E655-E662. doi: 10.1073 / pnas.1317903111 . PMID 24453215 .
  20. ^ Fund of the chemical industry, lecturer price
  21. ^ Karl Heinz Beckurts Prize Winner 2000
  22. ^ Laureates of the Tech Awards
  23. ↑ Winner of the Wilhelm Exner Medal ( Memento of the original from January 3, 2014 in the Internet Archive ) Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice. @1@ 2Template: Webachiv / IABot / www.wilhelmexner.org
  24. ^ European Grand Prix for Innovation, Prize Winners
  25. ↑ Founders of Molecular Partners receive the Swiss Technology Award
  26. ↑ Founders of Molecular Partners receive the de Vigier Prize ( Memento from March 12, 2016 in the Internet Archive )
  27. ^ ERC Advanced Grant, press release from the University of Zurich
personal data
SURNAME Plückthun, Andreas
ALTERNATIVE NAMES Plueckthun, Andreas; Pluckthun, Andreas
BRIEF DESCRIPTION German-Swiss biochemist
DATE OF BIRTH May 7, 1956
PLACE OF BIRTH Heidelberg