Antarctic phosphatase
| Antarctic phosphatase | ||
|---|---|---|
| Mass / length primary structure | 375 amino acids | |
| Cofactor | Zn 2+ | |
| Identifier | ||
| Gene name (s) | pho4 | |
| External IDs | ||
| Occurrence | ||
| Parent taxon | Antarctic bacteria TAB5 | |
The Antarctic phosphatase is a phosphatase , the removal of 5'- phosphate groups in DNA or RNA catalyzed. It was isolated from the Antarctic bacterium TAB5 in 2000 and is marketed because of its heat sensitivity.
Since fragments treated with Antarctic phosphatase no longer have the 5'-phosphoryl termini required by ligases , they cannot self-ligate. This property in cloning strategies can be used to create the vector background, i.e. H. the presence of unwanted vector molecules. Antarctic phosphatase can be quickly heat-inactivated at 65 ° C.
The enzyme belongs to the group of alkaline phosphatases , see also shrimp alkaline phosphatase and calf intestinal phosphatase .
literature
- Rina M, Pozidis C, Mavromatis K, Tzanodaskalaki M, Kokkinidis M, Bouriotis V: Alkaline phosphatase from the Antarctic strain TAB5. Properties and psychrophilic adaptations . In: Eur. J. Biochem. . 267, No. 4, February 2000, pp. 1230-8. PMID 10672035 .
Web links
- Scientific information from the manufacturer New England Biolabs ( Memento from August 21, 2012 in the Internet Archive )