Assay
The word assay (German: test, sample) comes from the English-speaking world and generally means an examination for the detection of certain substances (e.g. metals). In German, assay refers to a standardized reaction sequence for the detection of a substance with a specific method , especially in laboratory medicine and pharmaceutical research . In particular, molecular biological methods are meant.
There is no special feature that distinguishes an assay from other detection methods , at most that the occurrence of the name indicates the origin of the name or the method from the English-speaking area. In many cases, other words have been substituted for assay (e.g. Bradford test ).
Examples
Detection of DNA and its binding to proteins
Detection of RNA
Detection of protein
- BCA assay, see bicinchoninic acid
- Bradford test
- Lowry test
- Secretion assay
- ELISA
- Indirect immunoperoxidase assay
- Scintillation proximity assay
- Comet assay
- Immunoassay
Detection of viruses
- Plaque assay
- Radioimmunoassay (RIA)
- Enzyme-Linked Fluorescence Assay (ELFA)
- Electrochemical Luminescence Immunoassay (ECLIA)
See also
Individual evidence
- ↑ E. Karageorgou, V. Samanidou: Youden test application in robustness assays during method validation. In: Journal of chromatography. A. Volume 1353C, August 2014, pp. 131-139, ISSN 1873-3778 . doi : 10.1016 / j.chroma.2014.01.050 . PMID 24508395 .
- ↑ JL Colangelo: Integration of analytical techniques for protein biomarker assays. In: Advances in chromatography. Volume 52, 2014, pp. 55-78, ISSN 0065-2415 . PMID 24843905 .