Bicinchoninic acid

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Structural formula
Structural formula of bicinchoninic acid
General
Surname Bicinchoninic acid
other names
  • 2,2'-biquinoline-4,4'-dicarboxylic acid
  • 4,4'-dicarboxy-2,2'-biquinoline
Molecular formula C 20 H 12 N 2 O 4
Brief description

beige solid

External identifiers / databases
CAS number 1245-13-2
EC number 214-990-9
ECHA InfoCard 100,013,628
PubChem 71068
Wikidata Q386096
properties
Molar mass 344.33 g mol −1
Physical state

firmly

safety instructions
GHS labeling of hazardous substances
no GHS pictograms
H and P phrases H: no H-phrases
P: no P-phrases
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Bicinchoninsäure ( English b icin c honinic a cid, BCA ) is the common name for the chemical compound 2,2'-biquinoline-4,4'-dicarboxylic acid. It is a heterocyclic compound that consists of two quinoline molecules linked in the 2-position, each of which has carboxy groups in the 4-position. It is therefore a dicarboxylic acid .

properties

BCA reacts like biquinoline with monovalent copper ions to form a complex compound with a purple color.

Structural formula of the purple Cu + -bicinchoninic acid complex.

use

The BCA test is used in biochemistry for the quantitative, photometric or colorimetric determination of proteins (0.5 μg / mL - 1.5 mg / mL). Bivalent copper ions react quantitatively with protein to form monovalent copper ions. Together with bicinchoninic acid, these give a violet dye, the absorption of which can be evaluated photometrically at a wavelength of 562 nm.

Individual evidence

  1. ↑ a b c Data sheet bicinchoninic acid from Sigma-Aldrich , accessed on May 14, 2017 ( PDF ).Template: Sigma-Aldrich / name not given
  2. ↑ a b M. Holtzhauer: Biochemical Laboratory Methods , p. 10, 3rd edition, Springer Verlag, Berlin, 2009, ISBN 3-540-62435-X .
  3. ↑ PK Smith, et al. : Measurement of protein using bicinchoninic acid , in: Anal. Biochem. , 1985 , Vol. 150, pp. 76-85; PMID 3843705 ; doi : 10.1016 / 0003-2697 (85) 90442-7 , correction in Anal. Biochem. , 1987 , vol. 163, p. 279; doi : 10.1016 / 0003-2697 (87) 90125-4 .
  4. ↑ KJ Wiechelman, et al. : Investigation of the bicinchoninic acid protein assay: identification of the groups responsible for color formation , in: Anal. Biochem. , 1985 , Vol. 175, pp. 231-237; PMID 3245570 ; doi : 10.1016 / 0003-2697 (88) 90383-1 .
  5. ↑ CM Stoscheck: Quantitation of protein , in: Methods Enzymol. , 1990 , Vol. 182, pp. 50-68; PMID 2314256 .

Web links

Wiktionary: Bicinchoninsäure  - explanations of meanings, word origins, synonyms, translations