BHK-21 cells

from Wikipedia, the free encyclopedia
BHK-21 cells cultured on a flexible support

BHK-21 cells (from baby hamster kidney , baby hamster kidney) are cells of a cell line that emerged from the immortalized kidney cells of a day- old hamster . Among other things, they are used to produce recombinant proteins or to culture some viruses.

properties

BHK-21 cells are adherent cells and are fibroblasts from the cell line . Although they can be cultivated as suspension cells, they are then unstable and therefore not suitable for virus cultivation. Most of the time, clone 13 is used. They are propagated in cell culture and can be transfected . Furthermore, they can be transduced with adenovirus D, poliovirus 2, reovirus 3 and vesicular stomatitis virus (Indiana strain) . BHK-21 cells do not have an active reverse transcriptase .

history

BHK-21 cells were published in 1962 by IA Macpherson and MGP Stoker.

Individual evidence

  1. ^ A b F. Brown: Problems with BHK 21 cells. In: Developments in biological standardization. Volume 93, 1998, pp. 85-88, PMID 9737382 .
  2. ↑ C. Basilico, HK Meiss: Methods for selecting and studying temperature-sensitive mutants of BHK-21 cells. In: Methods in cell biology. Volume 8, 0 1974, pp. 1-22, PMID 4366305 .
  3. ^ I. Macpherson, M. Stoker: Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. In: Virology. Volume 16, February 1962, pp. 147-151, PMID 14468055 .
  4. ↑ M. STOKER, I. MACPHERSON: SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES. In: Nature. Volume 203, September 1964, pp. 1355-1357, PMID 14207308 .

Web links