Basement membrane-like matrix

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A basalmembranartige matrix ( English basement membrane-like matrix ) is a complex mixture of biomolecules , in the 3D cell culture and in tissue engineering is used as the basis for growth (matrix cell substrate). Brand names for basement membrane-like matrices are e.g. B. Matrigel , BME , EHS matrix .

properties

A basalmembranartige matrix is the purified secretion of the murine sarcoma - cell line Engelbreth-Holm-Swarm (EHS) cells and similar (as well as BME, Matrigel, EHS matrix) in its composition is partially the extracellular matrix of basement membranes of animal cells . It contains laminin , entactin , collagen and heparan sulfate - proteoglycans , among other things . The basement membrane-like matrix of EHS cells forms a hydrogel at 37 ° C , while it is liquid at 4 ° C. Compared to polylysine- coated cell culture bottles, Matrigel creates a modified three-dimensional cell structure.

In the cell culture of stem cells , feeding cells can partially be replaced by a basement membrane-like matrix. Basement membrane-like matrices are added to the tumor cells to be injected in oncological research when tumor models are established.

Individual evidence

  1. ^ A b C. S. Hughes, LM Postovit, GA Lajoie: Matrigel: a complex protein mixture required for optimal growth of cell culture. In: Proteomics. Volume 10, Number 9, May 2010, ISSN  1615-9861 , pp. 1886-1890, doi : 10.1002 / pmic.200900758 , PMID 20162561 .
  2. ^ A b G. Benton, HK Kleinman, J. George, I. Arnaoutova: Multiple uses of basement membrane-like matrix (BME / Matrigel) in vitro and in vivo with cancer cells. In: International journal of cancer. Journal international du cancer. Volume 128, Number 8, April 2011, ISSN  1097-0215 , pp. 1751-1757, doi : 10.1002 / ijc.25781 , PMID 21344372 .
  3. ↑ a b I. Arnaoutova, J. George, HK Kleinman, G. Benton: The endothelial cell tube formation assay on basement membrane turns 20: state of the science and the art. In: Angiogenesis. Volume 12, Number 3, 2009, ISSN  1573-7209 , pp. 267-274, doi : 10.1007 / s10456-009-9146-4 , PMID 19399631 .
  4. ↑ C. Xu, MS Inokuma, J. Denham, K. Golds, P. Kundu, JD Gold, MK Carpenter: Feeder-free growth of undifferentiated human embryonic stem cells. In: Nature Biotechnology . Volume 19, Number 10, October 2001, ISSN  1087-0156 , pp. 971-974, doi : 10.1038 / nbt1001-971 , PMID 11581665 .