Butane toxin

Potassium channel toxin alpha-KTx 12.1
other names	Butane toxin, BuTX
	Existing structure data : PDB 1C55 , PDB 1C56 , PDB 1WT7
Mass / length primary structure	40 amino acids , 4,514 Da
Identifier
External IDs	UniProt : P59936 ;

Butane toxin (BuTX) is a neurotoxin from the scorpions Tityus serrulatus , T. bahiensis , T. stigmurus and T. trivitattus .

properties

Butane toxin is a protein that binds to the voltage-controlled potassium channels Shaker B and Kv1.2 , as well as to the Ca ²⁺ -activated potassium channels K _Ca 1.1 and K _Ca 3.1 . The IC ₅₀ of butane toxin at Kv1.3 and at Kv1.2 are 0.55 nM and 6.19 nM, respectively. With a lower potency, it inhibits the potassium channels Kv1.1, Kv1.5, Kv1.6, Kv4.3, Kv7.1, Kv7.2, Kv7.4 and ERG / KCNH2. It does not inhibit the potassium channels Kv1.4, Kv2.1 and Kv3.1. Butane toxin also promotes the release of nitric oxide , interleukin-6 and TNF-alpha in J774.1 cells and has an inflammatory effect in mice. At the N terminus there is a disulfide bridge that is unusual for scorpion toxins . The LD ₅₀ after intravenous administration in mice is 826 μg / kg.

Occurrence

Butane toxin is made by three Brazilian and one Argentine species of scorpion. It was the first described scorpion toxin that occurs in several scorpion species of the same genus .

Web links

MeSH butane toxin

Individual evidence

↑ Potassium channel toxin alpha-KTx 12.1 . PubMed Protein Database. UniProtKB / Swiss-Prot: P59936.2.
↑ ^a ^b S. K. Holaday, BM Martin, PL Fletcher, NR Krishna: NMR solution structure of butantoxin. In: Archives of biochemistry and biophysics. Volume 379, Number 1, July 2000, pp. 18-27, doi : 10.1006 / abbi.2000.1858 , PMID 10864437 .
↑ S. Oyama, P. Pristovsek, L. Franzoni, TA Pertinhez, E. Schininà, C. gap, H. Rüterjans, EC Arantes, A. Spisni: Probing the pH-dependent structural features of alpha-KTx12.1, a potassium channel blocker from the scorpion Tityus serrulatus. In: Protein science: a publication of the Protein Society. Volume 14, number 4, April 2005, pp. 1025-1038, doi : 10.1110 / ps.041131205 , PMID 15772309 , PMC 2253457 (free full text).
↑ FV Coronas, AR de Roodt, TO Portugal, FZ Zamudio, CV Batista, F. Gómez-Lagunas, LD Possani: Disulfide bridges and blockage of Shaker BK (+) - channels by another butantoxin peptide purified from the Argentinean scorpion Tityus trivittatus. In: Toxicon: official journal of the International Society on Toxinology. Volume 41, Number 2, February 2003, pp. 173-179, PMID 12565736 .

[Protein_12.1-1] Potassium channel toxin alpha-KTx 12.1 . PubMed Protein Database. UniProtKB / Swiss-Prot: P59936.2.

[holaday-2] S. K. Holaday, BM Martin, PL Fletcher, NR Krishna: NMR solution structure of butantoxin. In: Archives of biochemistry and biophysics. Volume 379, Number 1, July 2000, pp. 18-27, doi : 10.1006 / abbi.2000.1858 , PMID 10864437 .

[oyama-3] S. Oyama, P. Pristovsek, L. Franzoni, TA Pertinhez, E. Schininà, C. gap, H. Rüterjans, EC Arantes, A. Spisni: Probing the pH-dependent structural features of alpha-KTx12.1, a potassium channel blocker from the scorpion Tityus serrulatus. In: Protein science: a publication of the Protein Society. Volume 14, number 4, April 2005, pp. 1025-1038, doi : 10.1110 / ps.041131205 , PMID 15772309 , PMC 2253457 (free full text).

[coronas-4] FV Coronas, AR de Roodt, TO Portugal, FZ Zamudio, CV Batista, F. Gómez-Lagunas, LD Possani: Disulfide bridges and blockage of Shaker BK (+) - channels by another butantoxin peptide purified from the Argentinean scorpion Tityus trivittatus. In: Toxicon: official journal of the International Society on Toxinology. Volume 41, Number 2, February 2003, pp. 173-179, PMID 12565736 .