CLED agar
A CLED agar plate after incubation , with no growth of microorganisms from a urine sample.
CLED agar with lactose- positive (yellow colored) and lactose-negative (blue colored) colonies.
CLED agar (Cystine Lactose Electrolyte Deficient Agar) is used in microbiology as a universal medium for microorganisms. The nutrient-rich and low-electrolyte nutrient medium is primarily used to cultivate bacteria from the urine.
Due to the lack of electrolytes, the swarming of Proteus is prevented. Lactose-fermenting bacteria form a yellow colony due to the indicator bromothymol blue , whereas non-lactose-fermenting bacteria form blue colonies.
| PH value | 7.3 |
| Peptone | 4 g / l |
| Meat extract | 3 g / l |
| Casein peptone | 4 g / l |
| Lactose | 10 g / l |
| L- cystine | 128 mg / l |
| Bromothymol blue | 20 mg / l |
| Agar | 15 g / l |
See also
Individual evidence
- ↑ Specification of CLED agar. (PDF) Carl Roth , August 29, 2016, accessed February 15, 2017 .