Muller-Hinton agar

Burkholderia pseudomallei colonies on Muller-Hinton agar after 72 hours of incubation.

Antibiogram of a tonsil smear of a dog, Mueller-Hinton agar. Only amoxicillin - clavulanic acid (AMC) and chloramphenicol (C) show an inhibitory effect.

The Müller-Hinton agar is a microbiological nutrient medium which is normally used to test antibiotic resistance and to grow Neisseria or Moraxella .

Composition::

• 30% beef infusion
• 1.75% hydrolyzed casein
• 0.15% starch
• 1.7% agar
• pH adjusted to neutral at 25 ° C.

5% sheep's blood is also added if you want to test streptococci , Campylobacter , Neisseria or other demanding germs. According to the new European standard EUCAST , sheep blood is no longer used, but horse blood and, as a further additive, nicotinamide adenine dinucleotide (NAD). With the addition of NAD, Haemophilus spp. on this agar.

See also

• Blood agar
• CLED agar
• Endo agar
• MacConkey agar
• Mannitol salt agar
• Sabouraud Dextrose Agar

Individual evidence

1. ↑ RM Atlas: Handbook of Microbiological Media . CRC Press, London 2004, ISBN 0849318181 , p. 1226.