COS cells

from Wikipedia, the free encyclopedia
COS-7 cells, magnified forty times

COS cells (of English. CV-1 in Origin, carrying SV40 , original CV-1, carries SV40 ') are cells of a cell line obtained from the immortalized CV-1 cells of an African green monkey ( Chlorocebus aethiops ) by transformation with a replication deficient simian virus 40 emerged. Among other things, they are used for the production of recombinant proteins and for the culture of some viruses .

properties

COS cells are adherently growing cells and the cell lineage is fibroblasts of the kidney. Most of the time, clones 1 or 7 are used. They are propagated in cell culture and can be transfected . Because of the immortalization with a replication-deficient simian virus 40, COS cells have the large T antigen . Therefore, vectors with an SV40 origin of replication are replicated in COS cells.

history

CV-1 cells were published in 1964. COS-1, COS-3 and COS-7 cells were published in 1981 by Y. Gluzman.

Individual evidence

  1. ^ A b Y. Gluzman: SV40-transformed simian cells support the replication of early SV40 mutants. In: Cell. Volume 23, Number 1, January 1981, pp. 175-182, PMID 6260373 .
  2. ↑ FC JENSEN, AJ GIRARDI, RV GILDEN, H. KOPROWSKI: INFECTION OF HUMAN AND SIMIAN TISSUE CULTURES WITH ROUS SARCOMA VIRUS. In: Proceedings of the National Academy of Sciences . Volume 52, July 1964, pp. 53-59, PMID 14192657 , PMC 300571 (free full text).