ctRNA

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Structure of the ctRNA p42d

ctRNA ( English counter-transcribed RNA , counter-transcribed RNA ') are RNA from bacteria , with which the number of plasmids in a bacterial cell is limited. The inhibition takes place when the plasmid replicates . Limiting replication by ctRNA is a mechanism for limiting the number of plasmid copies, e.g. B. to avoid excessive gene expression of the genes on the plasmid.

properties

ctRNA are non-coding ribonucleic acids , attached to the mRNA of repB bind and its Translation inhibit. RepB is a protein that initiates the rolling circle replication of plasmids. By inhibiting the transcription of RepB , the number of plasmid copies in the bacterium is reduced. The ctRNA-containing plasmids are therefore referred to as low copy number plasmids .

In Corynebacterium glutamicum , ctRNA serves as an antisense RNA . In Enterococcus faecium , the plasmid pJB01 contains the three open reading frames copA , repB and repC and on the opposite strand between copA and repB is the sequence of the pJB01-ctRNA, which binds to the three open reading frames and inhibits their translation. Further ctRNAs have been described in lactococci, Streptococcus thermophilus , and Rhizobium etli .

ctRNA, iterons and antisense RNA are bacterial mechanisms that limit the number of plasmid copies.

Individual evidence

  1. ↑ T. Venkova-Canova, M. Pátek, J. Nesvera: Control of rep gene expression in plasmid pGA1 from Corynebacterium glutamicum. In: Journal of bacteriology. Volume 185, Number 8, April 2003, pp. 2402-2409, ISSN  0021-9193 . PMID 12670963 . PMC 152619 (free full text).
  2. ↑ SW Kim, IS Jeong, EJ Jeong, JI Tak, JH Lee, SK Eo, HY Kang, JD Bahk: The terminal and internal hairpin loops of the ctRNA of plasmid pJB01 play critical roles in regulating copy number. In: Molecules and cells. Volume 26, Number 1, July 2008, pp. 26-33, ISSN  1016-8478 . PMID 18511887 .
  3. ^ AR Raha, WY Hooi, NS Mariana, S. Radu, NR Varma, K. Yusoff: DNA sequence analysis of a small cryptic plasmid from Lactococcus lactis subsp. lactis M14. In: plasmid. Volume 56, Number 1, July 2006, pp. 53-61, ISSN  0147-619X . doi : 10.1016 / j.plasmid.2006.03.003 . PMID 16675013 .
  4. ↑ P. Petrova, V. Miteva, JA Ruiz-Masó, G. del Solar: Structural and functional analysis of pt38, a 2.9kb plasmid of Streptococcus thermophilus yogurt strain. In: plasmid. Volume 50, Number 3, November 2003, pp. 176-189, ISSN  0147-619X . PMID 14597007 .
  5. ^ R. Cervantes-Rivera, C. Romero-López, A. Berzal-Herranz, MA Cevallos: Analysis of the mechanism of action of the antisense RNA that controls the replication of the repABC plasmid p42d. In: Journal of bacteriology. Volume 192, Number 13, July 2010, pp. 3268-3278, ISSN  1098-5530 . doi : 10.1128 / JB.00118-10 . PMID 20435728 . PMC 2897686 (free full text).