Enzyme histochemistry

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Enzyme histochemistry. NADH staining of a muscle biopsy with disorders of the oxidative network (light cutouts) in type 1 fibers.

The enzyme histochemistry is a histological method by which the activity of certain enzymes in slice preparations can be detected and localized. In this case, soluble substrate is applied to a tissue section , which (in the presence of a corresponding enzyme activity in the tissue) is converted into an insoluble dye. The localization and extent of the enzyme activity can then be detected under the microscope . Since tissue fixation is associated with a loss of enzyme activity, enzyme histochemical examinations can only be carried out on unfixed frozen sections

Typical areas of application in medicine are the diagnosis of muscle diseases (e.g. NADH and ATPase staining on muscle biopsies ) or Hirschsprung's disease ( acetylcholinesterase staining).

literature

  • Z. Lojda, R. Gossrau, TH Schiebler: Enzyme histochemistry. A laboratory manual. Springer Verlag, Berlin 1979, ISBN 3-540-09269-2 .