Fbx15
| Fbx15 | ||
|---|---|---|
| Properties of human protein | ||
| Mass / length primary structure | 510 amino acids | |
| Identifier | ||
| Gene names | FBXO15 (FBX15, MGC39671) | |
| External IDs | ||
Fbx15 (engl. F-box protein 15 ) is a protein , the characteristic of embryonic stem cells is formed. Fbx15 is used to isolate induced pluripotent stem cells (iPS cells).
properties
Fbx15 plays a role in ubiquitinylation , which is used to label and break down proteins in a cell. Fbx15 is used in the ubiquitin proteasome system for substrate recognition as part of the protein complex of the E3 ubiquitin ligase of the SCF type (SKP1-CUL1-F-box protein). Fbx15 binds to Skp1 and Cul1 .
Fbx15 is the gene FBXO15 coded, both as a marker protein or a marker gene also be used. In the process of generating iPS cells, somatic cells are reprogrammed in vitro so that they regain pluripotency and resemble embryonic stem cells. Since not all cells are reprogrammed, the successfully reprogrammed cells must be separated from the other cells.
“A selection system introduced into these somatic cells on the basis of an independent ES-cell-specific gene (Fbx15) should make it possible to isolate successfully reprogrammed cells. In this way it could be shown that the joint overexpression of 4 genes - the Oct4, Sox2, Klf4 and c-Myc genes - in the connective tissue cells is sufficient to generate ES cell-like cells. "
Individual evidence
- ↑ K. Takahashi, Shinya Yamanaka : Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors. In: Cell (2006), Vol. 126, No. 4, pp. 663-676. PMID 16904174 .
- ↑ K. Katayama, K. Noguchi, Y. Sugimoto: FBXO15 regulates P-glycoprotein / ABCB1 expression through the ubiquitin-proteasome pathway in cancer cells. In: Cancer Sci. (2013), Volume 104, No. 6, pp. 694-702. doi : 10.1111 / cas.12145 . PMID 23465077 .
- ↑ Werner A. Müller, Monika Hassel: Developmental and reproductive biology of humans and important model organisms. Springer, Berlin Heidelberg 2012 p. 101. ISBN 3-642-28383-7 limited preview in the Google book search