Fish test

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A golden orfe

As fish test various investigations are pollutants in waters or aqueous media referred.

acute toxicity

The OECD test 203 checks the acute toxicity of a test substance on fish.

The fish are exposed to the test substance in various concentrations for 96 hours. The mortalities are recorded after 24, 48 and 72 hours. This enables the dose-effect relationship ( LC50 ) to be calculated. The highest concentration at which all fish survive is called LC 0 and the lowest concentration at which all fish die is called LC 100 .

The regulation allows the following fish species: Brachydanio rerio (zebrafish), Pimephales promelas (minnow), Cyprinus carpio (carp), Poecilia reticulata (guppy), Lepomis macrochirus (sunfish) and Oncorhynchus mykiss (rainbow trout).

Sewage toxicity

The fish test according to DIN 38412-L31, also known as the Goldorfentest, is a test for the toxicity of wastewater. This biotest is carried out with gold orfe , a cultivated form of the Alands ( Leuciscus idus ). The aim of the animal experiment is to determine from which dilution of the wastewater all animals survive for 48 hours. The result is the smallest integer value of the dilution level of the wastewater (G F value). It was required by the Waste Water Ordinance to determine the toxicity of waste water to fish until the spring of 2004 and was replaced by the fish egg test on January 1, 2005 (also for the purpose of collecting fees under the Waste Water Tax Act). Eggs of the zebrafish are used, the determined value is referred to as G egg .

Water monitoring

In the Kerren current fish test (1971) gold orfe must swim against a current generated at periodic intervals and be monitored automatically. If the fish are so weakened (by pollutants ) that they can no longer maintain their swimming position, they will be carried away by the current. If the error messages accumulate due to the drifting of the golden orfe, an alarm is triggered. The current fish test has been used for monitoring in several bodies of water in Germany since 1972 and has gradually been replaced by the dynamic daphnia test . Today it is no longer permitted in Germany for reasons of animal welfare and its low sensitivity.

Web links

Individual evidence

  1. ↑ Fish test (acute toxicity) , hydrotox.de
  2. ↑ DIN 38412-31: 1989-03: Test procedure with aquatic organisms (group L) ; Determination of the non-acutely toxic effects of waste water on fish using dilution levels (L 31).
  3. ↑ Dynamic biotests in water monitoring ( memento of the original from February 3, 2017 in the Internet Archive ) Info: The @1@ 2Template: Webachiv / IABot / www.lanuv.nrw.de archive link was automatically inserted and not yet checked. Please check the original and archive link according to the instructions and then remove this notice. on the LANUV website, accessed on February 3, 2017.