HaeIII
| HaeIII | ||
|---|---|---|
| Mass / length primary structure | 318 amino acids | |
| Identifier | ||
| Gene name (s) | HaeIII (Rebase) | |
| External IDs | ||
| Enzyme classification | ||
| EC, category | 3.1.21.4 , ribonuclease | |
| Response type | Cleavage of double-stranded DNA | |
| Substrate | X-GGCC-Y (DNA) + H 2 O | |
| Products | X-GG + CC-Y | |
| Occurrence | ||
| Parent taxon | Haemophilus aegyptius | |
The enzyme HaeIII belongs to the restriction endonucleases and is able to cut DNA at specific points. HaeIII is a type II restriction enzyme and is the third restriction enzyme isolated from the bacterium Haemophilus aegyptius . The coding gene has been sequenced and cloned, which means it can be used for many applications in molecular biology. The molar mass of the enzyme is 37,126 Da .
Like many other restriction enzymes, HaeIII binds to sites in DNA that have a specific nucleotide sequence.
| Recognition sequence | Restriction cut |
|---|---|
5'-GGCC-3' 3'-CCGG-5' |
5'-GG CC-3' 3'-CC GG-5' |
When cutting the double-stranded DNA, a smooth cut without overhangs is created. The ends of the DNA fragments are therefore referred to as blunt ends .