Main field of view

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A main field of view ( HPF , high-power field ) is the section of a specimen visible through a microscope at a total of 400x magnification . Depending on the construction of the eyepiece , it has an area of ​​0.1 to 0.4 mm². In the case of quantitative use, the size of the main field of view should be specified or the results converted to mm², see the field number of the eyepiece.

This term is used e.g. B. in the diagnosis of histological sections , e.g. B. 10 mitoses on 50 main fields of view . For this purpose, a main field of vision is set, all mitoses are counted and the preparation is systematically shifted until 50 HPF have been counted. The number of mitoses then provides an indication of the malignancy of some types of cancer. In this application, the variable that is actually of interest is the number density in the volume of the sample, so that the layer thickness of the specimen also matters.

Individual evidence

  1. ↑ Charité Berlin : Note on the TNM classification (PDF; 24 kB)
  2. ↑ Christopher Fletcher et al. a .: Diagnosis of gastrointestinal stromal tumors. A consensus approach ( Memento of the original from March 27, 2014 in the Internet Archive ) Info: The archive link was automatically inserted and not yet checked. Please check the original and archive link according to the instructions and then remove this notice. (PDF; 395 kB) . In: Human Pathology , Vol. 33 (2002), Issue 5, pp. 459-465, ISSN 0046-8177 doi : 10.1053 / hupa.2002.123545  @1@ 2Template: Webachiv / IABot / www.heinrich-corless.net 
  3. ↑ Waldemar Hort (Ed.): Pathology of the endocardium, the coronary arteries and the myocardium . Springer, Berlin 2000, ISBN 3-540-65326-0 , p. 1001 .