Hin dIII
| Endonuclease HindIII | ||
|---|---|---|
|
||
| Surface model of the dimer with DNA according to PDB 2E52 | ||
| Mass / length primary structure | 300 amino acids | |
| Secondary to quaternary structure | Homodimer | |
| Cofactor | magnesium | |
| Identifier | ||
| Gene name (s) | HindIII | |
| External IDs | ||
| Enzyme classification | ||
| EC, category | 3.1.21.4 , restriction enzyme | |
| Response type | hydrolysis | |
| Substrate | AAGCTT (dDNA) | |
| Occurrence | ||
| Parent taxon | Haemophilus influenzae | |
HindIII is an enzyme that is used in molecular biology for the targeted cleavage of DNA . This enzyme belongs to the family of type II restriction endonucleases and was first obtained in 1970 from the bacterium Haemophilus influenzae . Its molecular mass is 35 kDa . After dimerization , HindIII cuts double-stranded DNA within the palindromic recognition sequence to form a 5 'overhang as follows:
| Recognition sequence | Restriction cut |
|---|---|
5'-AAGCTT-3' 3'-TTCGAA-5' |
5'-A AGCTT-3' 3'-TTCGA A-5' |
This formation of a 5 'overhang ( sticky ends ) comprising four nucleobases by HindIII can also be used in molecular biology to facilitate the linking ( ligation ) of DNA fragments.
Individual evidence
- ^ HO Smith and KW Wilcox: A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. Journal of Molecular Biology (1970) 51 (2): pp. 379-391 PMID 5312500