Light transmission aggregometry

The light transmission aggregometry (LTA) , also called the Born test after the first description , is a standard examination of the function of the blood platelets and a special form of the platelet aggregometry . It can be used to characterize a large number of functional properties of platelets. The examination, the interpretation of the findings and the assignment to a defined clinical picture are demanding.

functionality

The aggregation of the blood platelets is measured after the addition of an activator by determining the turbidity of the sample photometrically . The maximum optical density is reached in the resting state of the platelets, which are evenly distributed in the solution. The clumping of the blood platelets leads to a decrease in the turbidity in the course of the measurement due to the increased light transmission. This is measured continuously by observing the transmission of long-wave light and recording it in the form of a curve.

A few milliliters of platelet-rich citrate plasma , which is kept in motion and obtained from the centrifugation of whole blood, are required for the measurement .

Various substances can be used as agonists for activating the blood platelets, e.g. B. ADP , collagen , arachidonic acid or ristocetin . This allows a distinction to be made between different causes of the dysfunction of the blood platelets.

Individual evidence

↑ AWMF Guideline Archived Copy ( Memento of the original from February 22, 2016 in the Internet Archive ) Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice. @1@ 2
↑ Karsten Schrör: New platelet function inhibitors in clinics and practices . Schattauer Verlag, 2013, ISBN 978-3-7945-2926-1 , pp. 82 ( limited preview in Google Book search).
↑ Dietmar Fries, Werner Streif: Coagulation management in intensive care medicine . Springer-Verlag, 2014, ISBN 978-3-642-05004-6 , pp. 22 ( limited preview in Google Book search).

[1] AWMF Guideline Archived Copy ( Memento of the original from February 22, 2016 in the Internet Archive ) Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice. @1@ 2

[Karsten_Schrör-2] Karsten Schrör: New platelet function inhibitors in clinics and practices . Schattauer Verlag, 2013, ISBN 978-3-7945-2926-1 , pp. 82 ( limited preview in Google Book search).

[Dietmar_Fries,_Werner_Streif-3] Dietmar Fries, Werner Streif: Coagulation management in intensive care medicine . Springer-Verlag, 2014, ISBN 978-3-642-05004-6 , pp. 22 ( limited preview in Google Book search).