Loop-mediated isothermal amplification

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The loop-mediated isothermal amplification (LAMP, English for "loop-mediated isothermal amplification") is a method for the amplification (duplication) of DNA . It is a variant of isothermal DNA amplification .

properties

The LAMP uses a strand - dislocating DNA polymerase (e.g. Bst DNA polymerase or Bst 2.0 ) and runs at a constant temperature (isothermal). The reaction can be accelerated by adding polyethylene glycol (8,000 to 20,000 Dalton ). Since four to six primers bind to six to eight DNA sequences in the LAMP , the primer design is complex compared to other amplification methods and is therefore usually carried out on the computer. Analogous to RT-PCR can be obtained by addition of a reverse transcriptase , a reverse transcription are performed. The resulting DNA can be quantified analogously to qPCR . Analogous to multiplex PCR , several DNA sequences can be detected in parallel.

Alternative methods of amplifying DNA are e.g. B. the polymerase chain reaction , the multidisplacement amplification , the isothermal assembly , the recombinase polymerase amplification (RPA), nucleic acid sequence-based amplification ( NASBA ), the helicase-dependent amplification (HDA), the nicking enzyme amplification reaction (NEAR), the rolling circle replication (RCA). Further detection methods are e.g. B. Nicking endonuclease signal amplification ( NESA ) and nicking endonuclease assisted nanoparticle activation ( NENNA ), exonuclease-aided target recycling , junction or Y-probes , split DNAZyme and deoxyribozyme amplification , non-covalent DNA catalysis and the hybridization chain reaction ( HCR).

Individual evidence

  1. ↑ T. Notomi, H. Okayama, H. Masubuchi, T. Yonekawa, K. Watanabe, N. Amino, T. Hase: Loop-mediated isothermal amplification of DNA. In: Nucleic Acids Research . Volume 28, Number 12, June 2000, p. E63, PMID 10871386 , PMC 102748 (free full text).
  2. ↑ M. Fakruddin, KS Mannan, A. Chowdhury, RM Mazumdar, MN Hossain, S. Islam, MA Chowdhury: Nucleic acid amplification: Alternative methods of polymerase chain reaction. In: Journal of pharmacy & bioallied sciences. Volume 5, number 4, October 2013, pp. 245-252, doi: 10.4103 / 0975-7406.120066 , PMID 24302831 , PMC 3831736 (free full text).
  3. ^ NA Tanner, Y. Zhang, TC Evans: Simultaneous multiple target detection in real-time loop-mediated isothermal amplification. In: BioTechniques. Volume 53, number 2, August 2012, pp. 81-89, doi : 10.2144 / 0000113902 (currently unavailable) , PMID 23030060 .
  4. Jump up ↑ Y. Mori, H. Kanda, T. Notomi: Loop-mediated isothermal amplification (LAMP): recent progress in research and development. In: Journal of Infection and Chemotherapy . Volume 19, Number 3, June 2013, pp. 404-411, doi: 10.1007 / s10156-013-0590-0 , PMID 23539453 .
  5. ↑ J. Kim, CJ Easley: Isothermal DNA amplification in bioanalysis: strategies and applications. In: Bioanalysis. Volume 3, number 2, January 2011, pp. 227-239, doi: 10.4155 / bio.10.172 , PMID 21250850 .
  6. ↑ K. Nose, K. Nagamine, J. Tokuda, J. Takino, T. Hori: [Polyethylene glycol accelerates loop-mediated isothermal amplification (LAMP) reaction]. In: Yakugaku zasshi: Journal of the Pharmaceutical Society of Japan. Volume 133, Number 10, 2013, pp. 1121-1126, PMID 24088355 .
  7. ↑ K. Nagamine, T. Hase, T. Notomi: Accelerated reaction by loop-mediated isothermal amplification using loop primers. In: Molecular and cellular probes. Volume 16, Number 3, June 2002, pp. 223-229, PMID 12144774 .
  8. ↑ C. Torres, EA Vitalis, BR Baker, SN Gardner, MW Torres, JM Dzenitis: LAVA: an open-source approach to designing LAMP (loop-mediated isothermal amplification) DNA signatures. In: BMC Bioinformatics. Volume 12, 2011, p. 240, doi: 10.1186 / 1471-2105-12-240 , PMID 21679460 , PMC 3213686 (free full text).
  9. ^ KA Curtis, DL Rudolph, SM Owen: Rapid detection of HIV-1 by reverse-transcription, loop-mediated isothermal amplification (RT-LAMP). In: Journal of virological methods. Volume 151, number 2, August 2008, pp. 264-270, doi: 10.1016 / j.jviromet.2008.04.011 , PMID 18524393 .
  10. ↑ N. Tomita, Y. Mori, H. Kanda, T. Notomi: Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. In: Nature protocols. Volume 3, number 5, 2008, pp. 877-882, doi: 10.1038 / nprot.2008.57 , PMID 18451795 .
  11. ↑ ZK Njiru, AS Mikosza, T. Armstrong, JC Enyaru, JM Ndung'u, AR Thompson: Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense. In: PLoS Neglected Tropical Diseases. Volume 2, number 1, 2008, p. E147, doi: 10.1371 / journal.pntd.0000147 , PMID 18253475 , PMC 2238707 (free full text).
  12. ↑ H. Iseki, A. Alhassan, N. Ohta, OM Thekisoe, N. Yokoyama, N. Inoue, A. Nambota, J. Yasuda, I. Igarashi: Development of a multiplex loop-mediated isothermal amplification (mLAMP) method for the simultaneous detection of bovine Babesia parasites. In: Journal of microbiological methods. Volume 71, Number 3, December 2007, pp. 281-287, doi: 10.1016 / j.mimet.2007.09.019 , PMID 18029039 .
  13. ↑ EC Oriero, J. Jacobs, JP Van Geertruyden, D. Nwakanma, U. D'Alessandro: Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination. In: Journal of Antimicrobial Chemotherapy . [Electronic publication before going to press] September 2014, doi: 10.1093 / jac / dku343 , PMID 25223973 .
  14. ↑ L. Yan, J. Zhou, Y. Zheng, AS Gamson, BT Roembke, S. Nakayama, HO Sintim: Isothermal amplified detection of DNA and RNA. In: Molecular bioSystems. Volume 10, Number 5, May 2014, pp. 970-1003, doi: 10.1039 / c3mb70304e , PMID 24643211 .