Polynucleotides
Polynucleotides are linear (unbranched), depending on the source of 13 or 20 or more nucleotides composite biopolymers with changing nucleobases . In contrast, nucleic acid polymers with a smaller number of nucleotides are referred to as oligonucleotides . The nucleotides are linked via a 5 '→ 3' phosphodiester bond . Polynucleotides include nucleic acids , which are divided into ribonucleic acids (RNA) and deoxyribonucleic acids (DNA).
Applications
The sequence of nucleotides in a polynucleotide is determined by DNA sequencing (in the case of DNA), in the case of RNA a reverse transcription is carried out beforehand. A reproduction of a DNA polynucleotide is represented by a PCR-based method, or isothermal DNA amplification achieved with an RNA polynucleotide comprising a reverse transcription is carried out previously.
Individual evidence
- ^ MeSH polynucleotides
- ^ Robert C. King: A Dictionary of Genetics. OUP USA, 2013, ISBN 978-0-199-76644-4 , p. 368.
- ↑ Pei show Juo: Concise Dictionary of Biomedicine and Molecular Biology. CRC Press, 2001, ISBN 978-1-420-04130-9 , p. 880.