Primosome
| Parent |
| Replisome |
| Gene Ontology |
|---|
| QuickGO |
A protein complex that is involved in the initiation of DNA replication is called a primosome . Primosomes are only found in prokaryotes . In eukaryotes , other proteins take on this function.
construction
A single primosome is a complex of seven proteins:
task
The primosome is required for setting the RNA primers on the two single strands of the DNA to be replicated. The helicase splits the DNA strands, but the unwinding and protection against damage and breakage is carried out by gyrase-like proteins. SSb proteins then ensure that the strand remains open so that there is no new base pairing. They have a short-term insulating function, so to speak. Then primases synthesize the primers and as soon as the DNA polymerases attach, the elongation can begin. On the following strand, the primosome remains part of the replication complex, since new RNA primers are constantly required for the Okazaki fragments that arise here .
Individual evidence
- ↑ H. Nakai, V. Doseeva, JM Jones: Handoff from recombinase to replisome: insights from transposition. In: Proceedings of the National Academy of Sciences of the United States of America . Volume 98, Number 15, July 2001, pp. 8247-8254, doi : 10.1073 / pnas.111007898 . PMID 11459960 . PMC 37428 (free full text).
- ↑ YH Huang, CY Huang: Structural insight into the DNA-binding mode of the primosomal proteins PriA, PriB, and DnaT. In: BioMed research international . Volume 2014, 2014, p. 195162, doi : 10.1155 / 2014/195162 . PMID 25136561 . PMC 4129139 (free full text).