Pseudotyping
As pseudotyping (engl. Pseudotyping ) of the exchange which is the viral envelope proteins in the production of viral vectors , respectively. This creates pseudotype vectors. The envelope proteins of the virus are replaced by foreign viral envelope proteins. In this way, various changes in the vector can be generated, which among other things cause a change in the tropism compared to the host cells or an increased stability of the virus particle. The pseudotypes are not genetically determined.
Pseudotyping enables, for example, the investigation of specific properties of the virus envelope proteins. A frequently used coat protein is the glycoprotein G of the vesicular stomatitis virus (VSV), abbreviated VSV-G . This envelope protein enables the transduction of almost all cell types .
Individual evidence
- ↑ Example for the generation of pseudotype vectors in a working group of the MHH ( Memento of the original of September 27, 2007 in the Internet Archive ) Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice.
- ↑ C. Lévy, E. Verhoeyen, FL Cosset: Surface engineering of lentiviral vectors for gene transfer into gene therapy target cells. In: Current opinion in pharmacology. Volume 24, October 2015, pp. 79-85, doi : 10.1016 / j.coph.2015.08.003 , PMID 26298515 .
- ↑ J. Cronin, XY Zhang, J. Reiser: Altering the tropism of lentiviral vectors through pseudotyping. In: Current gene therapy. Volume 5, Number 4, August 2005, pp. 387-398, PMID 16101513 , PMC 1368960 (free full text).
