Quantitative immunoprecipitation combined with knock-down

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Quantitative immunoprecipitation combined with knock-down is a biochemical method for determining protein-protein interactions . It is a combination of SILAC , RNA interference (RNAi), co-immunoprecipitation and quantitative mass spectrometry . Since this method is based on SILAC and thus relies on auxotrophies for arginine or lysine , a variant with metabolic labeling with 15 N was developed.

Individual evidence

  1. ↑ M. Selbach, M. Mann: Protein interaction screening by quantitative immunoprecipitation combined with knockdown (QUICK). In: Nature methods. Volume 3, Number 12, December 2006, pp. 981-983, doi : 10.1038 / nmeth972 , PMID 17072306 .
  2. ↑ P. Braun, ME Cusick, M. Vidal: QUICKstep and GS-TAP: new moves for protein-interaction analysis. In: Nature methods. Volume 3, Number 12, December 2006, pp. 975-976, doi : 10.1038 / nmeth1206-975 , PMID 17117154 .
  3. ^ S. Schmollinger, D. Strenkert, V. Offeddu, A. Nordhues, F. Sommer, M. Schroda: A protocol for the identification of protein-protein interactions based on 15N metabolic labeling, immunoprecipitation, quantitative mass spectrometry and affinity modulation. In: Journal of visualized experiments: JoVE. Number 67, September 2012, p., Doi : 10.3791 / 4083 , PMID 23051728 , PMC 3490270 (free full text).