ROSA26

nucleic acid
General
Surname	ROSA26
other names	Gt (PINK) 26Sor; gene trap ROSA 26, Philippe Soriano;
Identifiers
GenBank	14910
properties
Taxon	Mus musculus

ROSA26 ( English Reverse Oriented Splice Acceptor, Clone 26 ) refers to a position in the genome of mice on chromosome 6, between the base positions 113067428 and 113077333.

properties

The ROSA26 position is characterized by a consistently strong transcription of inserted genes in all developmental stages and cell types . The modified gene locus ROSAβgeo26 in modified laboratory strains contains a β-galactosidase and a neomycin phosphotransferase as reporter genes in the ROSA26 gene locus . The Cre recombinase , among other things, is used for insertion (e.g. a gene knockin ) .

The name ROSAβgeo26 refers to the particular type of gene trapping vector used, which has a splice -Erkennungs sequence contains in the reverse orientation. The number 26 is the original number of the cell clone . The ROSA26 gene locus was first identified in 1991 by Philippe Soriano's group using gene trapping in mouse embryonic stem cells (ESC). In the meantime, more than 130 transgenic mouse lines have been produced in which different genes have been inserted at the position of ROSA26. The human homolog of ROSA26 has similar properties.

literature

B. Wilm, R. Muñoz-Chapuli: Tools and Techniques for Wt1-Based Lineage Tracing. In: Methods in molecular biology. Volume 1467, 2016, pp. 41-59, doi : 10.1007 / 978-1-4939-4023-3_4 , PMID 27417958 .
A. Kleinhammer, J. Deussing, W. Wurst, R. Kühn: Conditional RNAi in mice. In: Methods. Volume 53, Number 2, February 2011, pp. 142-150, doi : 10.1016 / j.ymeth.2010.08.003 , PMID 20705138 .

Individual evidence

↑ ROSA26 in the MGI database .
↑ ^a ^b Information on ROSA26 from Soriano Lab, Icahn School of Medicine at Mount Sinai .
↑ BP Zambrowicz, A. Imamoto, S. Fiering, LA Herzenberg, WG Kerr, P. Soriano: Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells. In: Proceedings of the National Academy of Sciences . Volume 94, Number 8, April 1997, pp. 3789-3794, PMID 9108056 , PMC 20519 (free full text).
^ G Friedrich, P Soriano: Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice . In: Genes & Development . 5, No. 9, 1991, pp. 1513-23. doi : 10.1101 / gad.5.9.1513 . PMID 1653172 .
^ S Casola: Mouse models for miRNA expression: The ROSA26 locus . In: Methods in Molecular Biology . 667, 2010, pp. 145-63. doi : 10.1007 / 978-1-60761-811-9_10 . PMID 20827532 .
↑ S. Irion, H. Luche, P. Gadue, HJ Fehling, M. Kennedy, G. Keller: Identification and targeting of the ROSA26 locus in human embryonic stem cells. In: Nature Biotechnology . Volume 25, Number 12, December 2007, pp. 1477-1482, doi : 10.1038 / nbt1362 , PMID 18037879 .

[1] ROSA26 in the MGI database .

[Soriano_Lab-2] Information on ROSA26 from Soriano Lab, Icahn School of Medicine at Mount Sinai .

[3] BP Zambrowicz, A. Imamoto, S. Fiering, LA Herzenberg, WG Kerr, P. Soriano: Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells. In: Proceedings of the National Academy of Sciences . Volume 94, Number 8, April 1997, pp. 3789-3794, PMID 9108056 , PMC 20519 (free full text).

[4] G Friedrich, P Soriano: Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice . In: Genes & Development . 5, No. 9, 1991, pp. 1513-23. doi : 10.1101 / gad.5.9.1513 . PMID 1653172 .

[5] S Casola: Mouse models for miRNA expression: The ROSA26 locus . In: Methods in Molecular Biology . 667, 2010, pp. 145-63. doi : 10.1007 / 978-1-60761-811-9_10 . PMID 20827532 .

[6] S. Irion, H. Luche, P. Gadue, HJ Fehling, M. Kennedy, G. Keller: Identification and targeting of the ROSA26 locus in human embryonic stem cells. In: Nature Biotechnology . Volume 25, Number 12, December 2007, pp. 1477-1482, doi : 10.1038 / nbt1362 , PMID 18037879 .