Recombinant DNA

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As recombinant DNA ( rDNA ) is an artificial DNA - molecule referred to, the in vitro , using genetic engineering methods ( restriction and ligation was assembled), new. The DNA can come from different organisms or have been synthesized in vitro , either chemically by means of oligonucleotide synthesis , or enzymatically by means of the polymerase chain reaction (PCR). A recombinant protein is made from recombinant DNA, but not all recombinant DNA automatically codes for a protein.

An example of recombinant DNA are plasmids that are first isolated from bacterial cells and into which a transgene from foreign organisms or from an artificial gene synthesis is then incorporated. See also vectors in genetic engineering .

Recombinant DNA was first created in 1973 by the Americans Herbert W. Boyer and Stanley Norman Cohen .

Individual evidence

  1. ↑ Cohen SN , Chang AC, Boyer HW, Helling RB (1973): Construction of biologically functional bacterial plasmids in vitro. In: Proc. Natl. Acad. Sci. USA 70 (11): 3240-3244. PMID 4594039 , PMC 427208 (free full text)

literature

  • Campbell, Neil A. (1997): Biology , Spectrum, pp. 401-403.