Satiety mutagenesis

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Saturation mutagenesis is a molecular biological method for mutagenesis . A gene is generated by artificial gene synthesis with all possible mutations in a small, delimited region of a gene . Cassette mutagenesis and oligo-directed mutagenesis are types of satiety mutagenesis. In contrast to random mutagenesis, a limited gene region is specifically modified. Saturation mutagenesis is also used for directed evolution .

Individual evidence

  1. ↑ RM Myers, LS Lerman, Tom Maniatis : A general method for saturation mutagenesis of cloned DNA fragments. In: Science (1985), Vol. 229, No. 4710, pp. 242-247. PMID 2990046 .
  2. ^ WA Lim, RT Sauer: Alternative packing arrangements in the hydrophobic core of lambda repressor. In: Nature (1989) Volume 339, No. 6219, pp. 31-36. PMID 2524006 .
  3. ↑ LW Chiang: Saturation mutagenesis by mutagenic oligonucleotide-directed PCR amplification (Mod-PCR). In: Methods Mol Biol. (1996), Vol. 57, pp. 311-321. PMID 8850017 .
  4. ↑ MT Reetz, D. Kahakeaw, J. Sanchis: Shedding light on the efficacy of laboratory evolution based on iterative saturation mutagenesis. In: Mol Biosyst. (2009), Vol. 5, No. 2, pp. 115-122. doi : 10.1039 / b814862g . PMID 19156255 .