TlTx1

Kappa theraphotoxin Tb1a
other names	Theraphotoxin-1, TlTx1
Mass / length primary structure	35 amino acids , 4,193 Da
Identifier
External IDs	UniProt : P83745 ;

TlTx1 (synonym Kappa-theraphotoxin-Tb1a ) is a protein and neurotoxin that is produced by the giant tarantula Theraphosa blondi ( synonym : T. leblondi ).

properties

Disulfide bridges of TlTx1

TlTx1 is formed in the venom glands, like TlTx2 and TlTx3 . It inhibits voltage-gated potassium channels of the type Kv4.2 / KCND2 with an IC ₅₀ of 193 nM by shifting the activation of the channel towards stronger depolarization . Presumably, TITx1 binds to the S3-S4 linker in the voltage-dependent protein domain of the channel. It is related to heteropodatoxins and phrixotoxins . It has three disulfide bridges .

The toxins TlTx1-3 can be distinguished by mass spectrometry and by Edman degradation .

Web links

MeSH TlTx1

Individual evidence

↑ J. Ebbinghaus, C. Legros, A. Nolting, C. Guette, ML Celerier, O. Pongs, R. Bähring: Modulation of Kv4.2 channels by a peptide isolated from the venom of the giant bird-eating tarantula Theraphosa leblondi . In: Toxicon: official journal of the International Society on Toxinology. Volume 43, Number 8, June 2004, pp. 923-932, doi : 10.1016 / j.toxicon.2003.12.012 , PMID 15208026 .
↑ ^a ^b Uniprot : P83745 (TX1_THEBL) .
↑ LD Rash, WC Hodgson: Pharmacology and biochemistry of spider venoms. In: Toxicon: official journal of the International Society on Toxinology. Volume 40, Number 3, March 2002, pp. 225-254, PMID 11711120 .
↑ C. Legros, ML Célérier, M. Henry, C. Guette: Nanospray analysis of the venom of the tarantula Theraphosa leblondi: a powerful method for direct venom mass fingerprinting and toxin sequencing. In: Rapid communications in mass spectrometry: RCM. Volume 18, number 10, 2004, pp. 1024-1032, doi : 10.1002 / rcm.1442 , PMID 15150824 .

[1] J. Ebbinghaus, C. Legros, A. Nolting, C. Guette, ML Celerier, O. Pongs, R. Bähring: Modulation of Kv4.2 channels by a peptide isolated from the venom of the giant bird-eating tarantula Theraphosa leblondi . In: Toxicon: official journal of the International Society on Toxinology. Volume 43, Number 8, June 2004, pp. 923-932, doi : 10.1016 / j.toxicon.2003.12.012 , PMID 15208026 .

[Uniprot-2] Uniprot : P83745 (TX1_THEBL) .

[3] LD Rash, WC Hodgson: Pharmacology and biochemistry of spider venoms. In: Toxicon: official journal of the International Society on Toxinology. Volume 40, Number 3, March 2002, pp. 225-254, PMID 11711120 .

[4] C. Legros, ML Célérier, M. Henry, C. Guette: Nanospray analysis of the venom of the tarantula Theraphosa leblondi: a powerful method for direct venom mass fingerprinting and toxin sequencing. In: Rapid communications in mass spectrometry: RCM. Volume 18, number 10, 2004, pp. 1024-1032, doi : 10.1002 / rcm.1442 , PMID 15150824 .