Cell adhesion test

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A cell adhesion test (synonymous with cell binding test ) is a biochemical and biophysical method for determining cell adhesion .

properties

Tissue cells form cell contacts with one another , which among other things bring about the cohesion of the respective tissue and cell-cell communication. In contrast, suspension cells (e.g. blood cells such as PBMC ) are usually present individually. Cell contacts can be determined using suitable measuring methods. With an atomic force microscope , individual cell pairs can be pulled apart and the forces required for this can be determined. By sucking in liquid with a micropipette , constant suction can be used to separate cell clusters. By centrifugation cells can be separated at a defined force. By fluorescent labeling of cells or an isotopic label (usually 51 Cr ) cell contacts can be detected.

The hemagglutination (for. Example, when influenza virus ), and the rosetting (z. B. at poxviruses or plasmodia ) are characteristic properties of some infectious agents, and are used for determining the basis of their cell adhesion.

Individual evidence

  1. ^ RJ Klebe: Isolation of a collagen-dependent cell attachment factor. In: Nature . Volume 250, Number 463, July 1974, ISSN  0028-0836 , pp. 248-251, PMID 4859375 .
  2. ↑ G. Sagvolden, I. Giaever, EO Pettersen, J. Feder: Cell adhesion force microscopy. In: Proceedings of the National Academy of Sciences of the United States of America . Volume 96, Number 2, January 1999, ISSN  0027-8424 , pp. 471-476, PMID 9892657 , PMC 15160 (free full text).
  3. ↑ J. Helenius, CP Heisenberg, HE Gaub, DJ Muller: Single-cell force spectroscopy. In: Journal of cell science. Volume 121, Pt 11 June 2008, ISSN  0021-9533 , pp. 1785-1791, doi : 10.1242 / jcs.030999 , PMID 18492792 .
  4. ↑ KL Sung, LA Sung, M. Crimmins, SJ Burakoff, S. Chien: Determination of junction avidity of cytolytic T cell and target cell. In: Science . Volume 234, Number 4782, December 1986, ISSN  0036-8075 , pp. 1405-1408, PMID 3491426 .
  5. ↑ RM Hochmuth: Micropipette aspiration of living cells. In: Journal of biomechanics. Volume 33, Number 1, January 2000, ISSN  0021-9290 , pp. 15-22, PMID 10609514 .
  6. ↑ CD Reyes, AJ García: A centrifugation cell adhesion assay for high-throughput screening of biomaterial surfaces. In: Journal of biomedical materials research. Part A. Volume 67, number 1, October 2003, ISSN  1549-3296 , pp. 328-333, doi : 10.1002 / jbm.a.10122 , PMID 14517892 .
  7. ↑ DM Lewin son, BJ Nickoloff, EC Butcher: A fluorometric approach to the quantitation of cell number with application to a cell adhesion assay. In: Journal of immunological methods. Volume 110, Number 1, May 1988, ISSN  0022-1759 , pp. 93-100, PMID 3131438 .
  8. ↑ CQ Schmidt, AT Kennedy, WH Tham: More than just immune evasion: Hijacking complement by Plasmodium falciparum. In: Molecular immunology. [Electronic publication before printing] March 2015, ISSN  1872-9142 , doi : 10.1016 / j.molimm.2015.03.006 , PMID 25816986 .