PBMC

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When PBMC ( acronym for germ .: Peripheral Blood Mononuclear Cell ; dt .: mononuclear peripheral blood cells') are mononuclear cells of the blood designates that a round nucleus possess. These are, for example, lymphocytes and monocytes . These cells play an important role in the immune system in fighting infection .

PBMC are often used in diagnostics as an indicator of existing infections, as a prognostic factor for the course of an HIV infection and in clinical research. PBMCs are also used very often in HIV research, as these also include CD4 + cells (the cells that are infected by the HIV virus ).

PBMC are by centrifugation through a hydrophilic colloid purified either from sucrose-epichlorohydrin copolymers ( Ficoll , Histopaque ) of silica gel ( Percoll , Sepracell ) from cross-linked dextrans ( Macrodex ) or gelatin ( Plasmagel exists).

literature

  • Kirsten Gehlhar: Immunological Methods in Medicine . Script available at [1] (PDF; 1.77 MB)
  • Sandro Conrad: Reactions of cells of the immune system to radiation with carbon ions and X-rays . ( PDF ; 7.3 MB)

Individual evidence

  1. ↑ D. English, BR Andersen: Single-step separation of red blood cells, granulocytes and mononuclear leukocytes on discontinuous density gradient of Ficoll-Hypaque. In: J. Immunol. Methods. (1974), Vol. 5, pp. 249-252. PMID 4427075 .
  2. ^ A. Ferrante, YH Thong: Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leukocytes from human peripheral blood by the Hypaque-Ficoll method. In: J. Immunol. Methods. (1980) Vol. 36, pp. 109-117.
  3. ↑ M. Slifkin, R. Cumbie: Comparison of the Histopaque-1119 method with the Plasmagel method for separation of blood leukocytes for cytomegalovirus isolation. In: J Clin Microbiol. (1992), Vol. 30 (10), pp. 2722-2724. PMID 1328282 ; PMC 270506 (free full text).
  4. ^ CV Paya, AD Wold, TF Smith: Detection of cytomegalovirus from blood leukocytes separated by sepracell-MN and Ficoll-Paque / Macrodex methods. In: J Clin Microbiol. (1988), Vol. 26 (10), pp. 2031-2033. PMID 2846635 ; PMC 266810 (free full text).