Sucrose epichlorohydrin copolymer

Structural formula
	Monomer units
General
Surname	Sucrose epichlorohydrin copolymer
other names	α- D -glucopyranoside, β- D -fructofuranosyl, polymer with 2- (chloromethyl) oxirane
CAS number	26873-85-8
Monomers / partial structures	Sucrose; Epichlorohydrin;
Type of polymer	Copolymer
Brief description	Powder, white to white with a pale yellow tinge
properties
Physical state	firmly
solubility	easily soluble in water
safety instructions
GHS labeling of hazardous substances	no GHS pictograms
	no GHS pictograms
H and P phrases	H: no H-phrases
	P: no P-phrases
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Sucrose-epichlorohydrin copolymer is a synthetically produced copolymer from sucrose and epichlorohydrin that dissolves easily in water. The molecule is highly branched and reacts neutrally in aqueous solution .

The copolymer is used in density gradient centrifugation in biochemical analysis.

Manufacturing

Ficoll is prepared from sucrose and epichlorohydrin copolymerized . The density of the copolymer can be influenced. A density of 1 to 1.2 g / ml is used for gradient centrifugation.

use

Sucrose-epichlorohydrin copolymer is used in biological and medical research and diagnostics for the isolation of mononuclear cells ( PBMC ) under the brand names Ficoll , Histopaque or Polysucrose . As a component of the sample buffer for agarose gels , it increases the density of the DNA -containing solution brought into the sample wells, so that it stays longer in the wells. Sucrose-epichlorohydrin copolymers have practically no osmotic activity due to their high molar mass ( Ficoll 400 : on average 400 kDa ) and are therefore more suitable than sucrose, in particular for density gradient centrifugation.

literature

Werner Luttmann, Kai Bratke, Michael Küpper, Daniel Myrtek: The Experimenter: Immunology . 2nd Edition. Spectrum Akademischer Verlag, Heidelberg 2006, ISBN 3-8274-1730-9

Individual evidence

↑ European Chemicals Agency: List of pre-registered substances
↑ ^a ^b Product information sheet Ficoll PM 400 at Sigma-Aldrich , accessed September 26, 2019.
↑ Description of Ficoll PM 70 at Sigma-Aldrich , accessed September 26, 2019.
↑ Template: CL Inventory / not harmonized There is not yet a harmonized classification for this substance . A labeling of α-D-glucopyranoside, β-D-fructofuranosyl, polymer with 2- (chloromethyl) oxirane in the Classification and Labeling Inventory of the European Chemicals Agency (ECHA), accessed on September 24, 2019, is reproduced from a self-classification by the distributor .
↑ Data sheet Ficoll PM70 / PM400 (PDF; 239 kB) from GE Healthcare

[ECHA-1] European Chemicals Agency: List of pre-registered substances

[Sigma2-2] Product information sheet Ficoll PM 400 at Sigma-Aldrich , accessed September 26, 2019.

[Sigma1-3] Description of Ficoll PM 70 at Sigma-Aldrich , accessed September 26, 2019.

[4] Template: CL Inventory / not harmonized There is not yet a harmonized classification for this substance . A labeling of α-D-glucopyranoside, β-D-fructofuranosyl, polymer with 2- (chloromethyl) oxirane in the Classification and Labeling Inventory of the European Chemicals Agency (ECHA), accessed on September 24, 2019, is reproduced from a self-classification by the distributor .

[5] Data sheet Ficoll PM70 / PM400 (PDF; 239 kB) from GE Healthcare