Sucrose epichlorohydrin copolymer
Structural formula | |||||||
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Monomer units | |||||||
General | |||||||
Surname | Sucrose epichlorohydrin copolymer | ||||||
other names |
α- D -glucopyranoside, β- D -fructofuranosyl, polymer with 2- (chloromethyl) oxirane |
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CAS number | 26873-85-8 | ||||||
Monomers / partial structures | |||||||
Type of polymer | |||||||
Brief description |
Powder, white to white with a pale yellow tinge |
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properties | |||||||
Physical state |
firmly |
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solubility |
easily soluble in water |
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safety instructions | |||||||
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As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions . |
Sucrose-epichlorohydrin copolymer is a synthetically produced copolymer from sucrose and epichlorohydrin that dissolves easily in water. The molecule is highly branched and reacts neutrally in aqueous solution .
The copolymer is used in density gradient centrifugation in biochemical analysis.
Manufacturing
Ficoll is prepared from sucrose and epichlorohydrin copolymerized . The density of the copolymer can be influenced. A density of 1 to 1.2 g / ml is used for gradient centrifugation.
use
Sucrose-epichlorohydrin copolymer is used in biological and medical research and diagnostics for the isolation of mononuclear cells ( PBMC ) under the brand names Ficoll , Histopaque or Polysucrose . As a component of the sample buffer for agarose gels , it increases the density of the DNA -containing solution brought into the sample wells, so that it stays longer in the wells. Sucrose-epichlorohydrin copolymers have practically no osmotic activity due to their high molar mass ( Ficoll 400 : on average 400 kDa ) and are therefore more suitable than sucrose, in particular for density gradient centrifugation.
literature
- Werner Luttmann, Kai Bratke, Michael Küpper, Daniel Myrtek: The Experimenter: Immunology . 2nd Edition. Spectrum Akademischer Verlag, Heidelberg 2006, ISBN 3-8274-1730-9
Individual evidence
- ↑ European Chemicals Agency: List of pre-registered substances
- ↑ a b Product information sheet Ficoll PM 400 at Sigma-Aldrich , accessed September 26, 2019.
- ↑ Description of Ficoll PM 70 at Sigma-Aldrich , accessed September 26, 2019.
- ↑ harmonized classification for this substance . A labeling of α-D-glucopyranoside, β-D-fructofuranosyl, polymer with 2- (chloromethyl) oxirane in the Classification and Labeling Inventory of the European Chemicals Agency (ECHA), accessed on September 24, 2019, is reproduced from a self-classification by the distributor . There is not yet a
- ↑ Data sheet Ficoll PM70 / PM400 (PDF; 239 kB) from GE Healthcare