5-carboxytetramethylrhodamine

Structural formula
General
Surname	5-carboxytetramethylrhodamine
other names	5-TAMRA
Molecular formula	C 25 H 22 N 2 O 5
External identifiers / databases
CAS number 91809-66-4 PubChem 2762602 ChemSpider 2043318 Wikidata Q27122704
properties
Molar mass	430.45 g mol −1
safety instructions
GHS labeling of hazardous substances no GHS pictograms H and P phrases H: no H-phrases P: no P-phrases
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

5-carboxytetramethylrhodamine ( 5-TAMRA ) is a red fluorescent dye from the group of rhodamines . It can be classified both in the group of triphenylmethane dyes and xanthene dyes . 5 ‑ TAMRA is used for fluorescent labeling of nucleic acids and proteins . The carboxy group can be converted into a reactive intermediate state with coupling reagents . Alternatively, the succinimidyl ester 5-carboxytetramethylrhodamine- N- succinimidyl ester ( 5-TAMRA-SE) can be used for marking. 5-TAMRA is used, among other things, for fluorescent substrates of enzymes , hybridization probes and FRET .

Individual evidence

1. ↑ ^{a b c} Data sheet 5-Carboxytetramethylrhodamine from Sigma-Aldrich , accessed on October 31, 2016 ( PDF ).
2. ↑ Maksim V. Kvach, Dmitry A. Tsybulsky, Vadim V. Shmanai, Igor A. Prokhorenko, Irina A. Stepanova, Vladimir A. Korshun: Current Protocols in Nucleic Acid Chemistry . John Wiley & Sons, Inc., 2001, ISBN 978-0-471-14270-6 , chapter Non-Nucleoside Phosphoramidites of Xanthene Dyes (FAM, JOE, and TAMRA) for Oligonucleotide Labeling , doi : 10.1002 / 0471142700.nc0455s52 , PMID 23512693 .
3. ↑ GH Lee, EJ Lee, SS Hah: TAMRA- and Cy5-labeled probe for efficient kinetic characterization of caspase-3. In: Analytical biochemistry . Volume 446, February 2014, pp. 22-24; doi: 10.1016 / j.ab.2013.10.013 , PMID 24144487 .