6-carboxy-4 ', 5'-dichloro-2', 7'-dimethoxyfluorescein- N- succinimidyl ester

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Structural formula
Structure of 6-carboxy-4 ′, 5′-dichloro-2 ′, 7′-dimethoxyfluorescein-N-succinimidyl ester
General
Surname 6-carboxy-4 ', 5'-dichloro-2', 7'-dimethoxyfluorescein- N- succinimidyl ester
other names

6-JOE-SE

Molecular formula C 27 H 17 Cl 2 NO 11
Brief description

red solid

External identifiers / databases
CAS number 113394-23-3
PubChem 44625907
ChemSpider 22504655
Wikidata Q27902946
properties
Molar mass 602.33 g mol −1
Physical state

firmly

solubility
  • slightly soluble in water
  • soluble in DMF and DMSO
safety instructions
GHS labeling of hazardous substances
no GHS pictograms
H and P phrases H: no H-phrases
P: no P-phrases
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

6-Carboxy-4 ', 5'-dichloro-2', 7'-dimethoxyfluorescein- N -succinimidyl ester ( 6-JOE-SE ) is a fluorescent dye from the group of fluoresceins . It is used for fluorescent labeling of nucleic acids for DNA sequencing . It is characterized by a high quantum yield and low sensitivity to the pH value .

Individual evidence

  1. ↑ a b AAT Bioquest: 6-JOE, SE ( Memento from November 1, 2016 in the Internet Archive )
  2. ↑ a b c d data sheet 6-Carboxy-4 ′, 5′-dichloro-2 ′, 7′-dimethoxyfluorescein N-hydroxysuccinimide ester from Sigma-Aldrich , accessed on October 31, 2016 ( PDF ).
  3. ↑ MV Kvach, DA Tsybulsky, VV Shmanai, IA Prokhorenko, IA Stepanova, VA Korshun: Non-nucleoside phosphoramidites of xanthene dyes (FAM, JOE, and TAMRA) for oligonucleotide labeling. In: Current protocols in nucleic acid chemistry. Chapter 4, March 2013, S. Unit 4.55, doi: 10.1002 / 0471142700.nc0455s52 , PMID 23512693 .