6-FAM-phosphoramidite

Structural formula
General
Surname	6-FAM-phosphoramidite
other names	6-FAM-amidite; [(3 ', 6'-Dipivaloylfluoresceinyl) -6-carboxamidohexyl] -1- O - (2-cyanoethyl) - ( N , N -diisopropyl) phosphoramidite; 2,2-Dimethylpropanoic acid 1,1 '- (6- {10- [bis (1-methylethyl) amino] -13-cyano-1-oxo-9,11-dioxa-2-aza-10-phosphatridec-1 -yl} -3-oxospiro [isobenzofuran-1 (3 H ), 9 ′ - (9 H ) xanthene] -3 ′, 6′-diyl) ester ( IUPAC );
Molecular formula	C 46 H 58 N 3 O 10 P
Brief description	pale yellow powder
External identifiers / databases
PubChem	16211911
Wikidata	Q245488
properties
Molar mass	843.94 g mol −1
Physical state	firmly
safety instructions
GHS labeling of hazardous substances	no GHS pictograms
	no GHS pictograms
H and P phrases	H: no H-phrases
	P: no P-phrases
	As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

6-FAM-phosphoramidite , also 6-FAM-amidite , is the phosphoramidite derivative of 6-carboxyfluorescein (FAM), a fluorescent dye that is used in the synthesis of oligonucleotides to generate hybridization probes for real-time PCR assays and thus is used for the quantitative detection of nucleic acid sequences .

6-FAM-phosphoramidite is a reporter dye that is covalently bound to the 5 'end of the probe, which is responsible for the color signal of the reaction. The excitation wavelength of 6-FAM-phosphoramidite is 488 nm, the emission wavelength 518 nm. A quencher dye is bound to the 3 'end of the probe (e.g. TAMRA). The Taq polymerase equipped with a 5 ' exonuclease activity removes the reporter dye from the DNA . After the complete synthesis of the PCR product, the quencher dye is also released. Due to the spatial separation, the reporter dye emits a fluorescence signal after excitation. If, on the other hand, the intact probe is excited, the quencher dye suppresses its fluorescence due to the spatial proximity to the reporter dye .

Individual evidence

↑ ^a ^b ^c data sheet 6-FAM-phosphoramidite from Sigma-Aldrich , accessed on May 22, 2017 ( PDF ).

Web links

Thomas A. Schild: Introduction to Real-Time Taqman PCR Technology . 7700 SDS workshop

[sigma-1] ta sheet 6-FAM-phosphoramidite from Sigma-Aldrich , accessed on May 22, 2017 ( PDF ).