ARS element

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An ARS element ( autonomously replicating sequence ) is a nucleotide sequence on the DNA of some yeasts that serves as the origin of replication to initiate replication .

properties

ARS elements were discovered in plasmids of the baker's yeast Saccharomyces cerevisiae , which, in addition to replicating the actual genome , were able to replicate themselves. It is now known that such sequences also serve as the ORI ( origin of replication ) starting point for replication in the actual genome . ARS elements are among others in the baker's yeast, in the fission yeast pombe Schizosaccharomyces and Candida guilliermondi ago. Since some of the ARS elements can only be used species-specifically for cloning purposes, consensus sequences have been developed for use in several species.

A typical single-cell ARS consists of around 150-200 nucleobases , whereby eukaryotic ARS are more complicated and, in mammals, significantly larger. Typically the ARS element is an A / T rich area.

Individual evidence

  1. ^ RK Clyne, TJ Kelly: Identification of autonomously replicating sequence (ARS) elements in eukaryotic cells. In: Methods (San Diego, Calif.). Volume 13, Number 3, November 1997, pp. 221-233, ISSN  1046-2023 . doi : 10.1006 / meth.1997.0522 . PMID 9441849 .
  2. ↑ E. Foureau, V. Courdavault, SM Navarro Gallón, S. Besseau, AJ Simkin, J. Crèche, L. Atehortùa, N. Giglioli-Guivarc'h, M. Clastre, N. Papon: Characterization of an autonomously replicating sequence in Candida guilliermondii. In: Microbiological research. Volume 168, Number 9, November 2013, pp. 580-588, ISSN  1618-0623 . doi : 10.1016 / j.micres.2013.04.006 . PMID 23726794 .
  3. ↑ I. Liachko, MJ Dunham: An autonomously replicating sequence for use in a wide range of budding yeasts. In: FEMS yeast research. Volume 14, Number 2, March 2014, pp. 364-367, ISSN  1567-1364 . doi : 10.1111 / 1567-1364.12123 . PMID 24205893 . PMC 3959236 (free full text).