Ficain
| Ficain | ||
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| Ribbon model of the isoform Ficain A, according to PDB 4YYQ | ||
| other names |
Ficin, Debricin, Higueroxyl delabarre |
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Available structural data : 4YYW , 4YYR , 4YYS , 4YYU , 4YYV |
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| Mass / length primary structure | 217 amino acids , 23,533 Da | |
| Isoforms | 4th | |
| Identifier | ||
| External IDs |
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| Enzyme classification | ||
| EC, category | 3.4.22.3 , cysteine protease | |
| Occurrence | ||
| Parent taxon | Ficus carica | |
Ficain (synonym : Ficin, Debricin, Higueroxyl delabarre ) is an enzyme from the group of cysteine proteases . It occurs in the milky sap of the real fig ( Ficus carica ) and Ficus glabrata .
properties
Ficain is an endopeptidase . It occurs in four isoforms (A to D). It is inhibited by cystatin with a dissociation constant of 5 × 10 −14 . The pH optimum of ficain is pH 7. In vitro , ficain requires the addition of thiols .
Applications
Ficain is used to determine blood group antigens . It hydrolyzes the blood group antigens M, N, S, Duffy a and Duffy b. It also enhances the detection of antigens of the blood groups Rh, Kidd, Lewis, I and P1. Ficain is also used as a meat tenderizer .
Web links
Individual evidence
- ^ IE Liener, B. Friedenson: Ficin . In: Methods Enzymol . 19, 1970, pp. 261-273. doi : 10.1016 / 0076-6879 (70) 19020-3 .
- ↑ K. Brocklehurst, F. Willenbrock, E. Salih: Cysteine proteinases . In: A. Neuberger, K. Brocklehurst (Eds.): New Comprehensive Biochemistry: Hydrolytic Enzymes . Elsevier, Amsterdam 1987, pp. 39-158.
- ^ A b Alan J. Barrett: Handbook of Proteolytic Enzymes. Academic Press, 2012, ISBN 978-0-123-82220-8 , p. 1878.