Hemolysis-in-gel test
The hemolysis-in-gel test ( HIG test ), and hemolysis-in-gel test , is a special method in the laboratory medicine to detect a subset of late occurring IgG - antibodies with high binding force ( affinity ). It is used, for example, as a confirmatory test for suspected rubella . Here are red blood cells from sheep blood with antigens of the rubella virus sensitized with 1% agarose mixed and poured into a 1.5 mm thick gel. Holes are then punched into the gel plate and filled with heat-inactivated patient serum for the test . After 20 hours, any antibodies that may be present in the patient's serum should have reacted with the antigens on the blood cells. Then the plate is covered with complementary guinea pig serum. Hemolysis now occurs in those blood cells that are loaded with antigen-antibody complexes . The diameter of the hemolytic reaction around the punch hole is proportional to the amount of antibodies that are present in the serum.
Individual evidence
- ↑ Hemolysis in Gel ( Memento from January 6, 2006 in the Internet Archive ). University of Veterinary Medicine, Vienna.