Hoesch test

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The Hoesch test is a search test with which porphobilinogen excretion in the urine can be determined. The qualitative test can be used for rapid diagnosis of symptoms of acute hepatic porphyrias such as acute intermittent porphyria (AIP), in which porphobilinogen (PBG) occurs as a preliminary stage of heme biosynthesis in the urine.

To carry out the test, two drops of fresh spontaneous urine are added to Ehrlich's reagent, a solution of dimethylaminobenzaldehyde in hydrochloric acid , and mixed. A positive test is given if the color is red. False positive results are possible due to indole-3-acetic acid , methyldopa , phenazopyridine hydrochloride and alcohol-related malnutrition. In the further diagnosis of porphyria, specific quantitative analyzes of the porphyrins in the urine should follow.

The Hoesch test, like the Watson-Schwartz test , which detects urobilinogen as well as porphobilinogen, can be replaced by semi-quantitative tests.

The test was described in 1947 by Kurt Hoesch (1890–1966), Professor of Internal Medicine at the Medical Academy in Düsseldorf .

literature

  • Hoesch test. In: Axel M. Gressner, Torsten Arndt (Hrsg.): Lexicon of the Medical Laboratory Diagnostics Vol. 1: Clinical Chemistry. Springer, 2006, ISBN 978-3-540-23660-3 , p. 588.
  • J. Lamon, TK With, AG Redeker: The Hoesch test: bedside screening for urinary porphobilinogen in patients with suspected porphyria. In: Clinical chemistry. Volume 20, Number 11, November 1974, pp. 1438-1440, ISSN  0009-9147 . PMID 4425514 .
  • CA Pierach, R. Cardinal, I. Bossenmaier, CJ Watson: Comparison of the Hoesch and the Watson-Schwartz tests for urinary porphobilinogen. In: Clinical chemistry. Volume 23, Number 9, September 1977, pp. 1666-1668, ISSN  0009-9147 . PMID 890911 .

Individual evidence

  1. ↑ a b Harald Renz: Integrative Clinical Chemistry and Laboratory Medicine. de Gruyter, 2004, ISBN 978-3-11-017367-3 , pp. 170f.
  2. ^ J. Ochai: Medical Laboratory Science: Theory and Practice. Tata McGraw Hill, 2003, ISBN 978-0-07-463223-9 , pp. 139f.
  3. ↑ a b Claus A. Pierach: Pophyrias . In: Edward T. Bope, Rick D. Kellerman, Robert E. Rakel: Conn's Current Therapy 2011. Saunders, 2010, ISBN 978-1-4377-0986-5 , p. 488.
  4. ↑ Peter Voswinckel: The black urine: from horror to laboratory parameters. Blackwell Wissenschaft, Berlin 1992, ISBN 3-89412-123-8 , p. 166.
  5. ↑ Kurt Hoesch: About the evaluation of urobilinogenuria and the reverse urobilinogen reaction. In: German Medical Weekly . 72, 1947, pp. 704-705, doi : 10.1055 / s-0028-1118813 .