Hypochromic effect

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The hypochromic effect occurs when the base pairs of the DNA are close together and therefore absorb little light .

The reason for the effect is the interactions of the π electrons at body temperature . When the DNA is heated, the double helix separates and the absorption area of ​​the incident light increases. This effect is relevant in the context of Fluometrie (see also fluorescence ), since by the effect of the change fluometrischen properties and so the guanine - cytosine content of DNA can be determined.

See also

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  • Thermodynamic investigation ( memento from June 29, 2007 in the Internet Archive ) script, Technical University of Darmstadt
  • Winter, Roland; Noll, Frank : Methods of Biophysical Chemistry, Teubner Study Books Chemistry (1st edition, Stuttgart 1998)